A quantitative study on the expression of synapsin II and N-ethylmaleimide-sensitive fusion protein in schizophrenic patients

被引：34
|
作者：
Imai, C
Sugai, T
Iritani, S
Niizato, K
Nakamura, R
Makifuchi, T
Kakita, A
Takahashi, H
Nawa, H [1 ]
机构：
[1] Niigata Univ, Inst Brain Res, Niigata 95021, Japan
[2] Matsuzawa Hosp, Setagaya Ku, Tokyo 1560057, Japan
[3] Saigata Hosp, Div Clin Res, Ogata, Niigata 9493193, Japan
[4] Niigata Univ, Inst Brain Res, Pathol & Brain Dis Res Ctr, Niigata 9518585, Japan
来源：
NEUROSCIENCE LETTERS | 2001年 / 305卷 / 03期
基金：
日本学术振兴会;
关键词：
DNA microarray; schizophrenia; RNA profiling; synapsin II; NSF; postmortem brain;
D O I：
10.1016/S0304-3940(01)01844-4
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The application of DNA array technology to schizophrenic studies enabled us to assess molecular features of this disease. The expression of synapsin II and N-ethylmaleimide-sensitive fusion protein (NSF) mRNAs is reported to decrease in the prefrontal cortex of these patients. We attempted to reproduce this result with two distinct approaches. With high quality samples, mRNA and protein levels for synapsin II and NSF were measured by real-time polymerase chain reaction and by immunoblotting. Both experiments led to the same conclusion: The expression of these presynaptic markers is not altered significantly in the prefrontal cortex of our schizophrenic samples, compared to that in control subjects. These observations suggest that the neurochemical impairments of synapses reported in schizophrenia are not evident for all presynaptic markers and needs to be re-evaluated at molecular levels. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码：185 / 188
页数：4
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