Development and characterization of novel tetra-, tri-, and dinucleotide microsatellite markers in rainbow trout (Oncorhynchus mykiss)

被引：72
作者：
Spies, IB
Brasier, DJ
O'Reilly, TL
Seamons, TR
Bentzen, P
机构：
[1] NOAA Fisheries, Alaska Fisheries Sci Ctr, Seattle, WA 98115 USA
[2] Univ Calif San Diego, La Jolla, CA 92093 USA
[3] Bedford Inst Oceanog, Dartmouth, NS B2Y 4A2, Canada
[4] Univ Washington, Marine Mol Biotechnol Lab, Seattle, WA 98105 USA
[5] Dalhousie Univ, Dept Biol, Halifax, NS B3H 4J1, Canada
来源：
MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 02期
关键词：
enrichment; genome mapping; microsatellites; Oncorhynchus mykiss; population genetics; rainbow trout;
D O I：
10.1111/j.1471-8286.2005.00900.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We discuss the development and characterization of 40 polymorphic rainbow trout (Oncorhynchus mykiss) microsatellite loci. We used enriched libraries to isolate 14 dinucleotide, seven trinucleodide, eight compound di/tetranucleotide, and 11 tetranucleotide loci. These markers will be useful for selective breeding via marker-assisted selection, population genetics studies, parentage analysis, and have already been used for genome mapping.
引用
收藏
页码：278 / 281
页数：4
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