Up-regulation of antioxidant enzymes and coenzyme Q10 in a human oral cancer cell line with acquired bleomycin resistance

被引：23

作者：

Yen, Hsiu-Chuan ^{[1
,2
]}

Li, Sin-Hua ^{[1
,2
,3
]}

Majima, Hideyuki J. ^{[4
,5
]}

Huang, Yu-Hsiang ^{[1
,2
]}

Chen, Chiu-Ping ^{[1
,2
]}

Liu, Chia-Chi ^{[1
,2
]}

Tu, Ya-Chi ^{[1
,2
]}

Chen, Chih-Wei ^{[1
,2
]}

机构：

[1] Chang Gung Univ, Dept Med Biotechnol & Lab Sci, Tao Yuan 333, Taiwan

[2] Chang Gung Univ, Grad Inst Med Biotechnol & Lab Sci, Tao Yuan 333, Taiwan

[3] Chang Gung Univ, Grad Inst Biomed Sci, Div Biochem & Cellular Mol Biol, Tao Yuan 333, Taiwan

[4] Kagoshima Univ, Grad Sch Med & Dent Sci, Dept Oncol, Kagoshima 890, Japan

[5] Kagoshima Univ, Dept Space Environm Med, Grad Sch Med & Dent Sci, Kagoshima 890, Japan

来源：

FREE RADICAL RESEARCH | 2011年 / 45卷 / 06期

关键词：

Bleomycin; resistance; coenzyme Q(10); antioxidant enzymes; reactive oxygen species; hydrogen peroxide; MANGANESE SUPEROXIDE-DISMUTASE; MITOCHONDRIAL-DNA; OXIDATIVE STRESS; MAMMALIAN-CELLS; OXYGEN; INDUCTION; APOPTOSIS; DAMAGE; MICE; LUNG;

D O I：

10.3109/10715762.2011.572969

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bleomycin (BLM) is an anti-cancer drug that can induce formation of reactive oxygen species (ROS). To investigate the association between up-regulation of antioxidant enzymes and coenzyme Q(10) (CoQ(10)) in acquired BLM resistance, one BLM-resistant clone, SBLM24 clone, was selected from a human oral cancer cell line, SCC61 clone. The BLM resistance of SBLM24 clone relative to a sub-clone of SCC61b cells was confirmed by analysis of clonogenic ability and cell cycle arrest. CoQ(10) levels and levels of Mn superoxide dismutase, glutathione peroxidase 1, catalase and thioredoxin reductase 1 were augmented in SBLM24 clone although there was also a mild increase in the expression of BLM hydrolase. Suppression of CoQ(10) levels by 4-aminobenzoate sensitized BLM-induced cytotoxicity. The results of suppression on enhanced ROS production by BLM and the cross-resistance to hydrogen peroxide in SBLM24 clone further demonstrated the development of adaptation to oxidative stress during the formation of acquired BLM resistance.

引用

页码：707 / 716

页数：10

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