Thin-layer chromatography of gallic acid, methyl gallate, pyrogallol, phloroglucinol, catechol, resorcinol, hydroquinone, catechin, epicatechin, cinnamic acid, p-coumaric acid, ferulic acid and tannic acid

被引：94

作者：

Sharma, OP ^{[1
]}

Bhat, TK ^{[1
]}

Singh, B ^{[1
]}

机构：

[1] Indian Vet Res Inst, Reg Stn, Palampur 176061, Himachal Prades, India

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 822卷 / 01期

关键词：

phenols; tannic acid; catechin; epicatechin; cinnamic acid; p-coumaric acid; ferulic acid;

D O I：

10.1016/S0021-9673(98)00490-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Six solvent systems of varying suitability are reported for the thin-layer chromatographic separation of simple phenolics acid related compounds such as gallic acid, methyl gallate, pyrogallol, phloroglucinol, catechol, resorcinol, hydroquinone, catechin, epicatechin, cinnamic acid, p-coumaric acid, ferulic acid and tannic acid. The solvent system chloroform-ethyl acetate-acetic acid (50:50:1) facilitated the separation of all the compounds except pyrogallol and ferulic acid; resorcinol and hydroquinone, which were in turn resolved in benzene-dioxane-acetic acid (85:15.1). Detection was carried out using iodine vapour, ferric chloride reagent, ferric chloride-ferricyanide reagent and vanillin-sulfuric acid reagent. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：167 / 171

页数：5

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