Characterization of microsatellite markers for Saxicola species

被引:1
|
作者
Mortega, Kim G. [1 ,2 ,3 ,4 ,5 ,6 ]
Horsburgh, Gavin J. [6 ]
Illera, Juan C. [7 ]
Dawson, Deborah A. [6 ]
机构
[1] Max Planck Inst Ornithol, Dept Migrat & Immunoecol, D-78315 Radolfzell am Bodensee, Germany
[2] Univ Konstanz, Dept Ornithol, D-78457 Constance, Germany
[3] Univ Glasgow, Inst Biodivers Anim Hlth & Comparat Med, Glasgow G12 8QQ, Lanark, Scotland
[4] Natl Museums Kenya, Dept Ornithol, Nairobi, Kenya
[5] Tanzania Wildlife Res Inst, Arusha, Tanzania
[6] Univ Sheffield, Dept Anim & Plant Sci, Sheffield S10 2TN, S Yorkshire, England
[7] Univ Oviedo, Res Unit Biodivers UO CSIC, Asturias, Spain
基金
英国自然环境研究理事会;
关键词
Microsatellite; Passerine; Predicted genome locations; Saxicola torquata; Sequence tandem repeats (STR); Songbird;
D O I
10.1007/s12686-014-0355-9
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
We characterized 28 unique African stonechat (Saxicola torquata) microsatellite loci. Seventeen loci characterized in 24 unrelated Saxicola torquata axillaris individuals sampled at Mount Meru, Tanzania displayed 2-26 alleles per locus and observed heterozygosities ranged from 0.29 to 0.92. Heterozygous females and sequence similarity suggested all 17 loci were autosomal. All markers also successfully amplified in nine different species ranging from Europe to Asia, including three endemic Island species. These microsatellite markers will be useful to assess the genetic diversity of the large and widely distributed genus Saxicola, a group comprising 11 recognized species with evidences of cryptic diversification. Several species show a narrow range distribution and are of conservation concern.
引用
收藏
页码:273 / 278
页数:6
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