Pyrococcus furiosus Argonaute-mediated nucleic acid detection

被引：104

作者：

He, Ruyi ^{[1
]}

Wang, Longyu ^{[1
]}

Wang, Fei ^{[1
]}

Li, Wenqiang ^{[1
]}

Liu, Yang ^{[1
]}

Li, Aitao ^{[1
]}

Wang, Yuan ^{[2
]}

Mao, Wuxiang ^{[1
]}

Zhai, Chao ^{[1
]}

Ma, Lixin ^{[1
]}

机构：

[1] Hubei Univ, Hubei Collaborat Innovat Ctr Green Transformat Bi, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn,Hubei Ke, Wuhan, Hubei, Peoples R China

[2] Hubei Univ Arts & Sci, Med Coll, Xiangyang, Hubei, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 88期

基金：

中国国家自然科学基金;

关键词：

DNA; DIAGNOSTICS; GENERATION; BIOMARKER;

D O I：

10.1039/c9cc07339f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We demonstrate that short single-stranded DNA generated by Pyrococcus furiosus Argonaute (PfAgo) can initiate a second round of cleavage. Based on this principle, we established a molecular diagnostic method, termed PfAgo-mediated Nucleic acid Detection (PAND). This method could detect DNA at attomolar sensitivities, distinguish single-nucleotide mutants and accomplish multiplexed detection.

引用

页码：13219 / 13222

页数：4

共 23 条

[1] CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity [J].

Chen, Janice S. ;

Ma, Enbo ;

Harrington, Lucas B. ;

Da Costa, Maria ;

Tian, Xinran ;

Palefsky, Joel M. ;

Doudna, Jennifer A. .

SCIENCE, 2018, 360 (6387) :436-+

[2] Next-generation diagnostics with CRISPR [J].

Chertow, Daniel S. .

SCIENCE, 2018, 360 (6387) :381-382

[3] KrasG12D induces EGFR-MYC cross signaling in murine primary pancreatic ductal epithelial cells [J].

Diersch, S. ;

Wirth, M. ;

Schneeweis, C. ;

Joers, S. ;

Geisler, F. ;

Siveke, J. T. ;

Rad, R. ;

Schmid, R. M. ;

Saur, D. ;

Rustgi, A. K. ;

Reichert, M. ;

Schneider, G. .

ONCOGENE, 2016, 35 (29) :3880-3886

[4] Programmable DNA-Guided Artificial Restriction Enzymes [J].

Enghiad, Behnam ;

Zhao, Huimin .

ACS SYNTHETIC BIOLOGY, 2017, 6 (05) :752-757

[5] Cell-Free DNA: An Upcoming Biomarker in Transplantation [J].

Gielis, E. M. ;

Ledeganck, K. J. ;

De Winter, B. Y. ;

Del Favero, J. ;

Bosmans, J. -L. ;

Claas, F. H. J. ;

Abramowicz, D. ;

Eikmans, M. .

AMERICAN JOURNAL OF TRANSPLANTATION, 2015, 15 (10) :2541-2551

[6] Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6 [J].

Gootenberg, Jonathan S. ;

Abudayyeh, Omar O. ;

Kellner, Max J. ;

Joung, Julia ;

Collins, James J. ;

Zhang, Feng .

SCIENCE, 2018, 360 (6387) :439-+

[7] Dehydrogenation of Formic Acid Catalyzed by a Ruthenium Complex with an N,N′-Diimine Ligand [J].

Guan, Chao ;

Zhang, Dan-Dan ;

Pan, Yupeng ;

Iguchi, Masayuki ;

Ajitha, Manjaly J. ;

Hu, Jinsong ;

Li, Huaifeng ;

Yao, Changguang ;

Huang, Mei-Hui ;

Ming, Shixiong ;

Zheng, Junrong ;

Himeda, Yuichiro ;

Kawanami, Hajime ;

Huang, Kuo-Wei .

INORGANIC CHEMISTRY, 2017, 56 (01) :438-445

[8] DNA-guided DNA cleavage at moderate temperatures by Clostridium butyricum Argonaute [J].

Hegge, Jorrit W. ;

Swarts, Daan C. ;

Chandradoss, Stanley D. ;

Cui, Tao Ju ;

Kneppers, Jeroen ;

Jinek, Martin ;

Joo, Chirlmin ;

van der Oost, John .

NUCLEIC ACIDS RESEARCH, 2019, 47 (11) :5809-5821

[9] Prokaryotic Argonaute proteins: novel genome-editing tools? [J].

Hegge, Jorrit W. ;

Swarts, Daan C. ;

van der Oost, John .

NATURE REVIEWS MICROBIOLOGY, 2018, 16 (01) :5-11

[10] Genotyping with CRISPR-Cas-derived RNA-guided endonucleases [J].

Kim, Jong Min ;

Kim, Daesik ;

Kim, Seokjoong ;

Kim, Jin-Soo .

NATURE COMMUNICATIONS, 2014, 5

← 1 2 3 →