Pyrococcus furiosus Argonaute-mediated nucleic acid detection

被引：94
|
作者：
He, Ruyi [1 ]
Wang, Longyu [1 ]
Wang, Fei [1 ]
Li, Wenqiang [1 ]
Liu, Yang [1 ]
Li, Aitao [1 ]
Wang, Yuan [2 ]
Mao, Wuxiang [1 ]
Zhai, Chao [1 ]
Ma, Lixin [1 ]
机构：
[1] Hubei Univ, Hubei Collaborat Innovat Ctr Green Transformat Bi, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn,Hubei Ke, Wuhan, Hubei, Peoples R China
[2] Hubei Univ Arts & Sci, Med Coll, Xiangyang, Hubei, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 88期
基金：
中国国家自然科学基金;
关键词：
DNA; DIAGNOSTICS; GENERATION; BIOMARKER;
D O I：
10.1039/c9cc07339f
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We demonstrate that short single-stranded DNA generated by Pyrococcus furiosus Argonaute (PfAgo) can initiate a second round of cleavage. Based on this principle, we established a molecular diagnostic method, termed PfAgo-mediated Nucleic acid Detection (PAND). This method could detect DNA at attomolar sensitivities, distinguish single-nucleotide mutants and accomplish multiplexed detection.
引用
收藏
页码：13219 / 13222
页数：4
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