Electrospun Fibers Improving Cellular Respiration via Mitochondrial Protection

被引：35

作者：

Chen, Wei ^{[1
,2
]}

Zheng, Dandan ^{[1
]}

Chen, Yanru ^{[3
]}

Ruan, Huitong ^{[2
]}

Zhang, Yuhui ^{[1
]}

Chen, Xiuyuan ^{[1
]}

Shen, Hongxing ^{[1
]}

Deng, Lianfu ^{[2
]}

Cui, Wenguo ^{[2
]}

Chen, Hao ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Dept Spine Surg, 160 Pujian Rd, Shanghai 200127, Peoples R China

[2] Shanghai Jiao Tong Univ, Shanghai Key Lab Prevent & Treatment Bone & Joint, Shanghai Inst Traumatol & Orthopaed, Ruijin Hosp,Sch Med, 197 Ruijin 2nd Rd, Shanghai 200025, Peoples R China

[3] Shanghai Jiao Tong Univ, Xinhua Hosp, Sch Med, Dept Neonatol, 1665 Kongjiang Rd, Shanghai 200082, Peoples R China

来源：

SMALL | 2021年 / 17卷 / 46期

基金：

中国国家自然科学基金;

关键词：

cellular respiration; diabetic fracture; Hif-1; mitochondrial function; tissue regeneration; ANGIOGENESIS; HYDROGEL;

D O I：

10.1002/smll.202104012

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Cellular respiration is the prerequisite for cell survival and functions, and mitochondrial function and microcirculation oxygen supply are essential for cellular respiration. However, in diabetic fracture, cellular respiration of bone marrow stem cells (BMSCs) is disrupted because of the dysfunction of mitochondria and microcirculation disorders. Here, the electrospun fibers of GelMA loaded with Hif-1 pathway activator (DFO) are constructed to improve the cellular respiration of BMSCs via protecting mitochondrial function and reconstructing microcirculation. The sequential process of electrospinning and UV crosslinking endowed the electrospun fibers with breathability and the biomechanical properties like the periosteum. In vitro biomolecular experiments showed that by crosslinking grafted polyethylene glycol acrylate liposomes loaded with DFO, the functional electrospun fibers can release DFO locally to activate Hif-1 in BMSCs, which can regulate the balance of Bcl-2/Bax to protect mitochondria and upregulate the expression of VEGF to reconstruct microcirculation. Animal experiments confirmed that the functional electrospun fibers can promote the recovery of diabetic fracture in vivo. These suggested that the functional electrospun fibers can improve cellular respiration for cell survival and functions of BMSCs. This study provides a new treatment strategy for diabetic fracture and other tissue regeneration on basis of cellular respiration improvement.

引用

页数：11

共 39 条

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