High-level fluoroquinolone-resistant clinical isolates of Escherichia coli overproduce multidrug efflux protein AcrA

被引：106
作者：
Mazzariol, A
Tokue, Y
Kanegawa, TM
Cornaglia, G
Nikaido, H
机构：
[1] Univ Calif Berkeley, Dept Cell & Mol Biol, Berkeley, CA 94720 USA
[2] Univ Verona, Dept Pathol, Microbiol Sect, I-37100 Verona, Italy
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2000年 / 44卷 / 12期
关键词：
D O I：
10.1128/AAC.44.12.3441-3443.2000
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Immunoblotting with antibody against AcrA, an obligatory component of the AcrAB multidrug efflux system, showed that this protein was overexpressed by greater than or equal to 170% in 9 of 10 clinical isolates of Esherichia coli with high-level ciprofloxacin resistance (MICs, greater than or equal to 32 mug/ml) but not in any of the 15 isolates for which the MIC was less than or equal to1 mug/ml.
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页码：3441 / 3443
页数：3
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共 18 条
[1]   Regulation of chromosomally mediated multiple antibiotic resistance: the mar regulon [J].
Alekshun, MN ;
Levy, SB .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (10) :2067-2075
[2]   Contributions of individual mechanisms to fluoroquinolone resistance in 36 Escherichia coli strains isolated from humans and animals [J].
Everett, MJ ;
Jin, YF ;
Ricci, V ;
Piddock, LJV .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1996, 40 (10) :2380-2386
[3]   AMPLIFIABLE RESISTANCE TO TETRACYCLINE, CHLORAMPHENICOL, AND OTHER ANTIBIOTICS IN ESCHERICHIA-COLI - INVOLVEMENT OF A NONPLASMID-DETERMINED EFFLUX OF TETRACYCLINE [J].
GEORGE, AM ;
LEVY, SB .
JOURNAL OF BACTERIOLOGY, 1983, 155 (02) :531-540
[4]   Genetic evidence for a role of parC mutations in development of high-level fluoroquinolone resistance in Escherichia coli [J].
Heisig, P .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1996, 40 (04) :879-885
[5]   Bacterial topoisomerases, anti-topoisomerases, and anti-topoisomerase resistance [J].
Hooper, DC .
CLINICAL INFECTIOUS DISEASES, 1998, 27 :S54-S63
[6]   TOPOISOMERASE-IV IS A TARGET OF QUINOLONES IN ESCHERICHIA-COLI [J].
KHODURSKY, AB ;
ZECHIEDRICH, EL ;
COZZARELLI, NR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (25) :11801-11805
[7]   Quinolone-resistant mutants of Escherichia coli DNA topoisomerase IV parC gene [J].
Kumagai, Y ;
Kato, J ;
Hoshino, K ;
Akasaka, T ;
Sato, K ;
Ikeda, H .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1996, 40 (03) :710-714
[8]   The local repressor AcrR plays a modulating role in the regulation of acrAB genes of Escherichia coli by global stress signals [J].
Ma, D ;
Alberti, M ;
Lynch, C ;
Nikaido, H ;
Hearst, JE .
MOLECULAR MICROBIOLOGY, 1996, 19 (01) :101-112
[9]   Identification of mar mutants among quinolone-resistant clinical isolates of Escherichia coli [J].
Maneewannakul, K ;
Levy, SB .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1996, 40 (07) :1695-1698
[10]   Multidrug efflux pumps of gram-negative bacteria [J].
Nikaido, H .
JOURNAL OF BACTERIOLOGY, 1996, 178 (20) :5853-5859
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