Tonicity-regulated gene expression

被引:20
作者
Ferraris, Joan D. [1 ]
Burg, Maurice B. [1 ]
机构
[1] NIH, NHLBI, US Dept HHS, Kidney & Electrolyte Metab Lab, Bethesda, MD USA
来源
OSMOSENSING AND OSMOSIGNALING | 2007年 / 428卷
关键词
D O I
10.1016/S0076-6879(07)28016-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Hypertonicity activates several different transcription factors, including TonEBP/OREBP, that in turn increase transcription of numerous genes. Hypertonicity elevates TonEBP/OREBP transcriptional activity by moving it into the nucleus, where it binds to its cognate DNA element (ORE), and by increasing its transactivational activity. This chapter presents protocols for measuring the transcriptional activity of TonEBP/OREBP and determining its subcellular localization, its binding to ORES, and activity of its transactivation domain.
引用
收藏
页码:279 / 296
页数:18
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