Electrospun gelatin/polycaprolactone nanofibrous membranes combined with a coculture of bone marrow stromal cells and chondrocytes for cartilage engineering

被引：78

作者：

He, Xiaomin ^{[1
]}

Feng, Bei ^{[1
,2
]}

Huang, Chuanpei ^{[1
]}

Wang, Hao ^{[1
]}

Ge, Yang ^{[1
]}

Hu, Renjie ^{[1
]}

Yin, Meng ^{[1
]}

Xu, Zhiwei ^{[1
]}

Wang, Wei ^{[1
]}

Fu, Wei ^{[1
,2
]}

Zheng, Jinghao ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Dept Pediat Cardiothorac Surg, Sch Med, Shanghai Childrens Med Ctr, Shanghai 200127, Peoples R China

[2] Shanghai Jiao Tong Univ, Inst Pediat Translat Med, Sch Med, Shanghai Childrens Med Ctr, Shanghai 200127, Peoples R China

来源：

INTERNATIONAL JOURNAL OF NANOMEDICINE | 2015年 / 10卷

关键词：

electrospinning; nanocomposite; cartilage tissue engineering; nanomaterials; stem cells; MESENCHYMAL STEM-CELLS; CHONDROGENIC DIFFERENTIATION; SCAFFOLDS; HYPERTROPHY; REGENERATION; PHENOTYPE; DEFECTS; CULTURE; GRAFTS; BMSCS;

D O I：

10.2147/IJN.S79461

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Electrospinning has recently received considerable attention, showing notable potential as a novel method of scaffold fabrication for cartilage engineering. The aim of this study was to use a coculture strategy of chondrocytes combined with electrospun gelatin/polycaprolactone (GT/PCL) membranes, instead of pure chondrocytes, to evaluate the formation of cartilaginous tissue. We prepared the GT/PCL membranes, seeded bone marrow stromal cell (BMSC)/chondrocyte cocultures (75% BMSCs and 25% chondrocytes) in a sandwich model in vitro, and then implanted the constructs subcutaneously into nude mice for 12 weeks. Gross observation, histological and immunohistological evaluation, glycosaminoglycan analyses, Young's modulus measurement, and immunofluorescence staining were performed postimplantation. We found that the coculture group formed mature cartilage-like tissue, with no statistically significant difference from the chondrocyte group, and labeled BMSCs could differentiate into chondrocyte-like cells under the chondrogenic niche of chondrocytes. This entire strategy indicates that GT/PCL membranes are also a suitable scaffold for stem cell-based cartilage engineering and may provide a potentially clinically feasible approach for cartilage repairs.

引用

页码：2089 / 2099

页数：11

共 49 条

[21] Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering [J].