RNA-Binding Protein RBP-P Is Required for Glutelin and Prolamine mRNA Localization in Rice Endosperm Cells

被引:47
|
作者
Tian, Li [1 ]
Chou, Hong-Li [1 ,2 ]
Zhang, Laining [1 ]
Hwang, Seon-Kap [1 ]
Starkenburg, Shawn R. [3 ]
Doroshenk, Kelly A. [1 ]
Kumamaru, Toshihiro [4 ]
Okita, Thomas W. [1 ]
机构
[1] Washington State Univ, Inst Biol Chem, Pullman, WA 99164 USA
[2] Penn State Univ, Ctr RNA Mol Biol, University Pk, PA 16802 USA
[3] Los Alamos Natl Lab, Los Alamos, NM 87545 USA
[4] Kyushu Univ, Fac Agr, Fukuoka, Fukuoka 8190395, Japan
来源
PLANT CELL | 2018年 / 30卷 / 10期
基金
美国国家科学基金会; 日本学术振兴会;
关键词
ENDOPLASMIC-RETICULUM; ARABIDOPSIS-THALIANA; GENE FAMILY; OSTUDOR-SN; TRANSPORT; IDENTIFICATION; EXPRESSION; OVEREXPRESSION; MATURATION; SUBDOMAIN;
D O I
10.1105/tpc.18.00321
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In developing rice (Oryza sativa) endosperm, mRNAs of the major storage proteins, glutelin and prolamine, are transported and anchored to distinct subdomains of the cortical endoplasmic reticulum. RNA binding protein RBP-P binds to both glutelin and prolamine mRNAs, suggesting a role in some aspect of their RNA metabolism. Here, we show that rice lines expressing mutant RBP-P mislocalize both glutelin and prolamine mRNAs. Different mutant RBP-P proteins exhibited varying degrees of reduced RNA binding and/or protein-protein interaction properties, which may account for the mislocalization of storage protein RNAs. In addition, partial loss of RBP-P function conferred a broad phenotypic variation ranging from dwarfism, chlorophyll deficiency, and sterility to late flowering and low spikelet fertility. Transcriptome analysis highlighted the essential role of RBP-P in regulating storage protein genes and several essential biological processes during grain development. Overall, our data demonstrate the significant roles of RBP-P in glutelin and prolamine mRNA localization and in the regulation of genes important for plant growth and development through its RNA binding activity and cooperative regulation with interacting proteins.
引用
收藏
页码:2529 / 2552
页数:24
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