Upregulation of Wilms' tumour gene 1 (WT1) in uterine sarcomas

被引：38

作者：

Coosemans, A.

Nik, S. Amini

Caluwaerts, S.

Lambin, S.

Verbist, G.

Van Bree, R.

Schelfhout, V.

de Jonge, E.

Dalle, I.

Jacomen, G.

Cassiman, J. J.

Moerman, Ph.

Vergote, I.

Amant, F.

机构：

[1] Katholieke Univ Leuven, Inst Canc, UZ Gasthuisberg, Louvain, Belgium

[2] Univ Hosp Gent, Dept Pathol, Ghent, Belgium

[3] Ziekenhuis Oost Limburg, Dept Obstet & Gynaecol, Ghent, Belgium

[4] AZ St Lucas, Dept Pathol, Brugge, Belgium

[5] Sint Maarten Hosp, Dept Pathol, Duffel, Belgium

[6] Katholieke Univ Leuven, UZ Gasthuisberg, Dept Pathol, Louvain, Belgium

来源：

EUROPEAN JOURNAL OF CANCER | 2007年 / 43卷 / 10期

关键词：

Wilms' tumour gene 1; WT1; sarcoma; uterus; cytoplasmic expression;

D O I：

10.1016/j.ejca.2007.04.008

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Aim: Overexpression of Wilms' tumour gene (WT1) has been proven in several tumours. Previous research of our group on the cell cycle of uterine leiomyosarcoma (LMS) and carcinosarcoma (CS) suggested a possible role for WT1. We therefore intended to further explore the expression pattern of \WT1 in uterine sarcomas. Methods: 27 CS, 38 LMS, 15 endometrial stromal sarcomas (ESS) and seven undifferentiated sarcomas (US) were collected. WT1 expression was evaluated by immunohistochemistry (IHC) in 87 samples, by RT-PCR (m-RNA expression) in 23 random selected samples and by Western blotting in 12 samples, separating cytoplasmic and nuclear proteins. A pilot study to detect mutations (exons 7-10) was performed on eight samples. Results: IHC showed WT1 positivity in 12/27 CS, 29/38 LMS, 7115 ESS and 4/7 US. All-but-one sample had a positive RT-PCR. All Western blottings were positive with more cytoplasmic expression in 9/12 cases. No mutations were found. Conclusions: WT1 is overexpressed in uterine sarcomas. Since increased levels of mRNA determine the biological role, WT1 might contribute to uterine sarcoma tumour biology. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：1630 / 1637

页数：8

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