Possibility of paclitaxel as an alternative radiosensitizer to 5-fluorouracil for colon cancer

被引：25

作者：

Hiro, Junichiro ^{[1
]}

Inoue, Yasuhiro ^{[1
]}

Toiyama, Yuji ^{[1
]}

Yoshiyama, Shigeyuki ^{[1
]}

Tanaka, Koji ^{[1
]}

Mohri, Yasuhiko ^{[1
]}

Miki, Chikao ^{[1
]}

Kusunoki, Masato ^{[1
]}

机构：

[1] Mie Univ, Grad Sch Med, Inst Life Sci, Div Reparat Med,Dept Gastrointestinal & Pediat Su, Tsu, Mie 5148507, Japan

来源：

ONCOLOGY REPORTS | 2010年 / 24卷 / 04期

关键词：

5-fluorouracil; colon cancer; paclitaxel; radiosensitivity; PHARMACOKINETIC MODULATING CHEMOTHERAPY; DNA-DAMAGE CHECKPOINT; CELL LUNG-CANCER; RADIATION SENSITIZER; CARCINOMA-CELLS; IN-VITRO; ADJUVANT RADIOTHERAPY; RANDOMIZED-TRIAL; ONCOLOGY-GROUP; RECTAL-CANCER;

D O I：

10.3892/or_0000095

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

To evaluate the feasibility of paclitaxel (PTX) radiosensitization for colon cancer, we investigated the cytotoxic and G2/M checkpoint protein (Chk 1, Wee 1, Bub 1. MAD2) effects of 5-fluorouracil (5-FU) or PTX combined with radiation in the human colon cancer cell line LoVo. Cytotoxicity and radiocytotoxicity were evaluated for each drug by the WST-8 colorimetric assay. The IC20 for each drug was determined as a cytotoxic concentration from a survival curve. LoVo cells were exposed to the IC20 of each drug for 24 h and then irradiated. Expressions of the G2/M checkpoint proteins were confirmed by Western blot analysis. Cytotoxicity was induced by 5-FU or PTX alone in a time-and dose-dependent manner. The IC20 of PTX caused higher radiosensitivity than the IC20 of 5-FU (P<0.05). Western blot analysis revealed different expression patterns of the G2/M checkpoint proteins between 5-FU and PTX pretreatments. 5-FU combined with radiation tended to decrease the expressions of all G2/M checkpoint proteins in a time-dependent manner. PTX combined with radiation maintained high expressions of Chk 1 and MAD2 proteins for 24 h post-radiation and, in particular. MAD2 protein was strongly induced by PTX with high-dose radiation. PTX showed higher radio-sensitization than 5-FU for the colon cancer cell line LoVo and may be an alternative radiosensitizer to 5-FU in the clinical setting.

引用

页码：1029 / 1034

页数：6