The beneficial effects of 18β-glycyrrhetinic acid on the experimental autoimmune encephalomyelitis (EAE) in C57BL/6 mouse model

被引：16
作者：
Kamisli, Suat [1 ]
Ciftci, Osman [2 ]
Taslidere, Asli [3 ]
Turkmen, Nese Basak [4 ]
Ozcan, Cemal [1 ]
机构：
[1] Univ Inonu, Fac Med, Dept Neurol, Malatya, Turkey
[2] Univ Pamukkale, Fac Med, Dept Pharmacol, Denizli, Turkey
[3] Univ Inonu, Fac Med, Dept Histol & Embryol, Malatya, Turkey
[4] Univ Inonu, Fac Pharm, Dept Pharmaceut Toxicol, Malatya, Turkey
来源：
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY | 2018年 / 40卷 / 04期
关键词：
EAE; 18 beta-glycyrrhetinic acid; C57BL/6; multiple sclerosis; TNF-alpha; EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS; GLOBAL CEREBRAL ISCHEMIA/REPERFUSION; MULTIPLE-SCLEROSIS; OXIDATIVE STRESS; NEURONAL DAMAGE; CELL-LINE; MICE; THERAPY; GLYCYRRHIZIN; PATHOGENESIS;
D O I：
10.1080/08923973.2018.1490318
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Aim: The aim of this study was to investigate the beneficial effects of 18 beta-glycyrrhetinic acid (GA) on the experimental allergic encephalomyelitis (EAE) in C57BL/6 mice. GA is a natural substance found in the root of licorice and is used in traditional Chinese medicine. It has many pharmacological activities such as antioxidant, anti-inflammatory, and anti-cancer effects. Materials and methods: A total of 40 C57BL/6 mice were divided equally into four groups: (1) Control, (2) EAE, (3) GA and (4) GAthornEAE. 14 days after induction of EAE with MOG35-55 and pertussis toxin, mice were treated with GA at doses of 100 mg/kg/day for 7 days intraperitoneally. Results: To our results, oxidative stress and lipid peroxidations (elevated TBARS levels, decreased GPx, SOD, CAT, and GSH levels) were significantly (p<. 01) increased, causing EAE in brain tissue. Also, histopathological damage (Caspase-3 and IL-17 activity, p <=.01) and cytokine levels (TNF-alpha and IL-1 beta, p<. 01) were induced with EAE in mice brain tissue. On the other hand, GA treatment significantly (p<. 01) reversed oxidative histological and immunological alterations caused by EAE. Conclusions: In conclusion, the GA treatment can protect the brain tissue against EAE in mice with its antioxidant and anti-inflammatory properties.
引用
收藏
页码：344 / 352
页数：9
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