Structural and Biochemical Features of Human Serum Albumin Essential for Eukaryotic Cell Culture

被引：166

作者：

Mishra, Vibhor ^{[1
]}

Heath, Richard J. ^{[1
]}

机构：

[1] St Jude Childrens Res Hosp, Prot Prod Facil, Shared Resource Ctr, Memphis, TN 38105 USA

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2021年 / 22卷 / 16期

关键词：

human serum albumin; cell culture; ligand binding; FATTY-ACID-BINDING; CHEMICAL CROSS-LINKING; SUSPENDED ANIMAL-CELLS; CONFORMATIONAL-CHANGES; PYRIDOXAL; 5-PHOSPHATE; AGITATED BIOREACTORS; DAMAGE MECHANISMS; CRYSTAL-STRUCTURE; FUSION PROTEIN; PLANT PEPTONES;

D O I：

10.3390/ijms22168411

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Serum albumin physically interacts with fatty acids, small molecules, metal ions, and several other proteins. Binding with a plethora of bioactive substances makes it a critical transport molecule. Albumin also scavenges the reactive oxygen species that are harmful to cell survival. These properties make albumin an excellent choice to promote cell growth and maintain a variety of eukaryotic cells under in vitro culture environment. Furthermore, purified recombinant human serum albumin is mostly free from impurities and modifications, providing a perfect choice as an additive in cell and tissue culture media while avoiding any regulatory constraints. This review discusses key features of human serum albumin implicated in cell growth and survival under in vitro conditions.

引用

页数：16

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