Enhancing linalool production by engineering oleaginous yeast Yarrowia lipolytica

被引：85
作者：
Cao, Xuan [1 ]
Wei, Liu-Jing [1 ]
Lin, Jia-Yu [1 ]
Hua, Qiang [1 ,2 ]
机构：
[1] East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China
[2] Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2017年 / 245卷
基金：
中国国家自然科学基金;
关键词：
Linalool; Yarrowia lipolytica; Linalool synthase; MVA pathway; SACCHAROMYCES-CEREVISIAE; DIPHOSPHATE SYNTHASE; EXPRESSION; LYCOPENE;
D O I：
10.1016/j.biortech.2017.06.105
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
In this study, stepwise increases in linalool production were obtained by combining metabolic engineering and process optimization of an unconventional oleaginous yeast Yarrowia lipolytica. The linalool synthetic pathway was successfully constructed by heterologously expressing a codon-optimized linalool synthase gene from Actinidia arguta in Y. lipolytica. To enhance linalool productivity, key genes involved in the mevalonate pathway were overexpressed in different combinations. Moreover, the overexpression of mutant ERG20(F88W-N119W) gene resulted in further linalool production. A maximum linalool titre of 6.96 +/- 0.29 mg/L was achieved in shake flasks, which was the highest level ever reported in yeasts. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1641 / 1644
页数：4
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