Mimicking Paracrine TGFβ1 Signals during Myofibroblast Differentiation in 3D Collagen Networks

被引：21
作者：
Ansorge, Michael [1 ]
Sapudom, Jiranuwat [1 ]
Chkolnikov, Marina [1 ]
Wilde, Martin [1 ]
Anderegg, Ulf [2 ]
Moeller, Stephanie [3 ]
Schnabelrauch, Matthias [3 ]
Pompe, Tilo [1 ]
机构：
[1] Univ Leipzig, Inst Biochem, Johannisallee 21-23, D-04103 Leipzig, Germany
[2] Univ Klinikum Leipzig, Dept Dermatol Venereol & Allergol, D-04103 Leipzig, Germany
[3] INNOVENT eV, Biomat Dept, Prussingstr 27B, D-07745 Jena, Germany
来源：
SCIENTIFIC REPORTS | 2017年 / 7卷
关键词：
TRANSFORMING GROWTH-FACTOR-BETA-1; MIGRATION; HEPARIN; GROWTH; FIBROBLASTS; GRADIENTS; AFFINITY;
D O I：
10.1038/s41598-017-05912-x
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
TGF1 is a key regulator for induction of tissue remodeling after dermal wounding. We present a model of paracrine delivery of TGF beta 1 for differentiation of dermal fibroblasts based on a fibrillar 3D collagen matrix and embedded TGF beta 1 releasing microparticles. We found differentiation into myofibroblasts was achieved in a TGF beta 1 dependent manner at much lower doses than systemic delivery. This effect is accounted to the slow and sustained TGF beta 1 release mimicking paracrine cell signals.
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页数：8
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