Insertion of type O-conserved neutralizing epitope into the foot-and-mouth disease virus type Asia1 VP1 G-H loop: effect on viral replication and neutralization phenotype

被引：14
作者：
Wang, Haiwei [1 ,2 ]
Xue, Mei [1 ]
Yang, Decheng [1 ]
Zhou, Guohui [1 ]
Wu, Donglai [1 ,2 ]
Yu, Li [1 ]
机构：
[1] Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Div Livestock Infect Dis, Harbin 150001, Peoples R China
[2] Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Key Lab Vet Publ Hlth,Minist Agr, Harbin 150001, Peoples R China
来源：
JOURNAL OF GENERAL VIROLOGY | 2012年 / 93卷
关键词：
MAJOR ANTIGENIC SITE; IMMUNODEFICIENCY-VIRUS; MONOCLONAL-ANTIBODY; CAPSID PROTEIN; INTEGRIN ALPHA(V)BETA(3); PROTECTIVE IMMUNITY; VACCINE; RECEPTOR; BINDING; CATTLE;
D O I：
10.1099/vir.0.040253-0
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Previously, we finely mapped the neutralizing epitopes recognized by foot-and-mouth disease virus (FMDV) type Asial-specific mAb 3E11 and FMDV type O-specific mAb 8E8. In this study, we engineered recombinant FMDVs of the serotype Asia1 (rFMDVs) displaying the type O-neutralizing epitope recognized by the mAb 8E8. These epitope-inserted viruses were genetically stable and exhibited growth properties that were similar to those of their parental virus. Importantly, the recombinant virus rFMDV-C showed neutralization sensitivity to both FMDV type Asia1 and type O mAbs, as well as to polyclonal antibodies. These results indicated that this epitope-inserted virus has the potential to induce neutralizing antibodies against both FMDV type Asial and type O. Our results demonstrated that the G-H loop of FMDV type Asial effectively displays the protective neutralizing epitopes of other FMDV serotypes, making this an attractive approach for the design of novel FMDV vaccines.
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页码：1442 / 1448
页数：7
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