Purification of Toxoplasma gondii oocysts by cesium chloride gradient

被引：29

作者：

Dumètre, A ^{[1
]}

Dardé, ML ^{[1
]}

机构：

[1] Fac Med, UPRES EA 3174, F-87025 Limoges, France

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2004年 / 56卷 / 03期

关键词：

cat; cesium chloride; oocyst; purification; Toxoplasma gondii;

D O I：

10.1016/j.mimet.2003.11.020

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the use of a cesium chloride (CsCl) gradient as an improvement for the purification of Toxoplasma gondii oocysts from concentrated suspensions. After concentration by sucrose flotation, this technique gives a >96% recovery of very pure unsporulated or sporulated oocysts, but requires "fresh" oocysts (: 10 weeks of age). This material is suitable for biochemical and immunological analyses of environmentally resistant T gondii oocysts. (C) 2004 Elsevier B.V. All rights reserved.

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收藏

页码：427 / 430

页数：4

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