A Method for Detecting Circulating Tumor Cells Based on the Measurement of Single-Cell Metabolism in Droplet-Based Microfluidics

被引：104

作者：

Del Ben, Fabio ^{[1
,2
]}

Turetta, Matteo ^{[4
]}

Celetti, Giorgia ^{[3
]}

Piruska, Aigars ^{[3
]}

Bulfoni, Michela ^{[4
]}

Cesselli, Daniela ^{[4
]}

Huck, Wilhelm T. S. ^{[3
]}

Scoles, Giacinto ^{[4
]}

机构：

[1] Univ Trieste, Grad Sch Nanotechnol, Via Valerio 2, Trieste, Italy

[2] CRO Aviano, Dept Clin Pathol, Via F Gallini 2, I-33081 Aviano, PN, Italy

[3] Radboud Univ Nijmegen, Inst Mol & Mat, Heyendaalseweg 135, NL-6525 AJ Nijmegen, Netherlands

[4] Azienda Osped Univ Udine, Dept Med & Biol Sci, Inst Pathol Anat, I-33100 Udine, Italy

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2016年 / 55卷 / 30期

关键词：

cancer; circulating tumor cells; metabolism; microfluidics; picodroplets; CLINICAL-SIGNIFICANCE; CANCER; SURVIVAL; MICRODROPLETS; PROGRESSION; GLYCOLYSIS; ENRICHMENT; DYNAMICS; PH;

D O I：

10.1002/anie.201602328

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The number of circulating tumor cells (CTCs) in blood is strongly correlated with the progress of metastatic cancer. Current methods to detect CTCs are based on immunostaining or discrimination of physical properties. Herein, a label-free method is presented exploiting the abnormal metabolic behavior of cancer cells. A single-cell analysis technique is used to measure the secretion of acid from individual living tumor cells compartmentalized in microfluidically prepared, monodisperse, picoliter (pL) droplets. As few as 10 tumor cells can be detected in a background of 200 000 white blood cells and proof-of-concept data is shown on the detection of CTCs in the blood of metastatic patients.

引用

页码：8581 / 8584

页数：4

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