Noninvasive fetal genotyping of human platelet antigen-1a

被引：58

作者：

Scheffer, P. G. ^{[1
,2
]}

Soussan, A. Ait ^{[1
,2
]}

Verhagen, O. J. H. M. ^{[1
,2
]}

Page-Christiaens, G. C. M. L. ^{[3
]}

Oepkes, D. ^{[4
]}

de Haas, M. ^{[1
,2
]}

van der Schoot, C. E. ^{[1
,2
]}

机构：

[1] Sanquin Res Amsterdam, Dept Expt Immunohaematol, NL-1006 AD Amsterdam, Netherlands

[2] Univ Amsterdam, Acad Med Ctr, Landsteiner Lab, NL-1105 AZ Amsterdam, Netherlands

[3] Univ Med Ctr Utrecht, Div Perinatol & Gynaecol, Utrecht, Netherlands

[4] Leiden Univ, Med Ctr, Dept Obstet, Leiden, Netherlands

来源：

BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY | 2011年 / 118卷 / 11期

关键词：

Alloimmune thrombocytopenia; cell-free DNA; fetal or neonatal alloimmune thrombocytopenia; human platelet antigen-1a; maternal plasma; noninvasive prenatal diagnosis; NEONATAL ALLOIMMUNE THROMBOCYTOPENIA; MATERNAL PLASMA;

D O I：

10.1111/j.1471-0528.2011.03039.x

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

We describe a reliable noninvasive fetal human platelet antigen (HPA)-1a genotyping assay on a real-time polymerase chain reaction (PCR) platform using cell-free fetal DNA isolated from maternal blood. Nonspecific amplification of maternal cell-free DNA is overcome by pre-PCR digestion of the cell-free DNA with the Msp1 restriction enzyme. Noninvasive fetal HPA-1a genotyping offers a safe method for alloimmunised pregnant women to determine whether their fetus is at risk of fetal or neonatal alloimmune thrombocytopenia (FNAIT) and whether interventions to prevent intracranial haemorrhage are required. The availability of this test is relevant to the ongoing debate on screening pregnancies for HPA-1a-mediated FNAIT.

引用

页码：1392 / 1395

页数：4

共 6 条

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