Bacterial spores as time capsules for cloned genes

被引：0

作者：

Tanooka, H

机构：

[1] Natl Canc Ctr, Res Inst, Tokyo 1040045, Japan

[2] Cent Res Inst Elect Power Ind, Komae, Tokyo 2018511, Japan

来源：

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES | 2003年 / 79卷 / 09期

关键词：

bacterial spore; plasmid; cloned gene; gene preservation;

D O I：

10.2183/pjab.79B.290

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Spores of bacteria Bacillus subtilis were examined for their potential for storing cloned genes in the dry state at ambient temperature for long years. A model plasmid used in this study is pTE22, which is 8.7 Kb in size and carries test Escherichia coli plasmid pBR322 in a backbone B. subtilis plasmid pHW1. After 14.8 years of storage of spores containing pTE22, plasmids recovered from the germinated spores maintained the antibiotics resistance and intact size of the restriction fragments.

引用

页码：290 / 291

页数：2

共 11 条

[1] NUCLEOTIDE-SEQUENCE AND FUNCTIONAL MAP OF PE194, A PLASMID THAT SPECIFIES INDUCIBLE RESISTANCE TO MACROLIDE, LINCOSAMIDE, AND STREPTOGRAMIN TYPE-B ANTIBIOTICS [J].