Diabetes and exposure to peritoneal dialysis solutions alter tight junction proteins and glucose transporters of rat peritoneal mesothelial cells

被引:25
作者
Debray-Garcia, Yazmin [1 ]
Sanchez, Elsa I. [1 ]
Rodriguez-Munoz, Rafael [1 ]
Venegas, Miguel A. [2 ]
Velazquez, Josue [1 ]
Reyes, Jose L. [1 ]
机构
[1] Natl Polytech Inst CINVESTAV IPN, Ctr Res & Adv Studies, Dept Physiol Biophys & Neurosci, Mexico City 07360, DF, Mexico
[2] RHK Technol Inc, Imaging Future Nanosci Staff Sci, 1050 East Maple Rd, Troy, MI 48083 USA
关键词
Peritoneal dialysis; Tight junctions; Epithelial to mesenchymal transition; Oxidative stress; Transepithelial electrical resistance; IN-VIVO; MESENCHYMAL TRANSITION; ANTIOXIDANT RESPONSE; EX-VIVO; EXPRESSION; MEMBRANE; MORTALITY; TRANSDIFFERENTIATION; MECHANISMS; SENESCENCE;
D O I
10.1016/j.lfs.2016.07.018
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Aim: To evaluate alterations in tight junction (TJ) proteins and glucose transporters in rat peritoneal mesothelial cells (RPMC) from diabetic rats and after treatment with peritoneal dialysis solutions (PDS) in vitro. Methods: Diabetes was induced in female Wistar rats by streptozotocin (STZ)-injection. Twenty-one days after STZ-injection, peritoneal thickness and mesothelial cell morphology were studied by light microscopy and microvilli length and density by atomic force microscopy. RPMC were obtained from healthy and diabetic rats. Mesothelial phenotype, evaluated by cytokeratin and pan-cadherin, epithelial to mesenchymal transition (EMT), evaluated by alpha-smooth muscle action (alpha-SMA) and vimentin, TJ proteins, claudins-1 and -2, and occludin, and glucose transporters, sodium and glucose co-transporters (SGLT)-1 and -2 and facilitative glucose transporters (GLUT)-1 and -2 were analyzed. Also, transepithelial electrical resistance (TER) was measured. Oxidative stress was estimated by measuring reactive oxygen species production, and protein carbonylation, receptor for advanced glycation end products (RAGE), nuclear factor erythroid related factor-2 (Nrf-2), and expression of antioxidant enzymes. Key findings: Peritoneal damage was present 21 days after STZ-injection. Diabetes induced changes in TJ and glucose transporters in RPMC together with decreased TER. RPMC from diabetic rats showed oxidative stress, which was enhanced by exposure to PDS. In addition, RPMC from diabetic rats showed early EMT. Significance: To our knowledge, this is the first study that shows changes in TJ proteins and glucose transporters of RPMC from diabetic rats. All these alterations might explain the increased peritoneal permeability observed in diabetic patients undergoing peritoneal dialysis. (C) 2016 Elsevier Inc All rights reserved.
引用
收藏
页码:78 / 89
页数:12
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