The dynamic state of protein turnover: It's about time

被引：117

作者：

Hinkson, Izumi V. ^{[1
]}

Elias, Joshua E. ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem & Syst Biol, Stanford, CA 94305 USA

来源：

TRENDS IN CELL BIOLOGY | 2011年 / 21卷 / 05期

基金：

美国国家科学基金会;

关键词：

GENOME-WIDE ANALYSIS; MASS-SPECTROMETRY; DEGRADATIVE RATES; CELL-CULTURE; AMINO-ACIDS; IN-VIVO; LIQUID-CHROMATOGRAPHY; STATISTICAL-MODEL; BODY PROTEINS; HALF-LIFE;

D O I：

10.1016/j.tcb.2011.02.002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The continual destruction and renewal of proteins that maintain cellular homeostasis has been rigorously studied since the late 1930s. Experimental techniques for measuring protein turnover have evolved to measure the dynamic regulation of key proteins and now, entire proteomes. In the past decade, the proteomics field has aimed to discover how cells adjust their proteomes to execute numerous regulatory programs in response to specific cellular and environmental cues. By combining classical biochemical techniques with modern, high-throughput technologies, researchers have begun to reveal the synthesis and degradation mechanisms that shape protein turnover on a global scale. This review examines several recent developments in protein turnover research, emphasizing the combination of metabolic labeling and mass spectrometry.

引用

页码：293 / 303

页数：11

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