Monitoring Campylobacter in the poultry production chain - From culture to genes and beyond

被引:28
作者
Josefsen, Mathilde H. [1 ]
Bhunia, Arun K. [2 ]
Engvall, Eva Olsson [3 ]
Fachmann, Mette S. R. [1 ]
Hoorfar, Jeffrey [1 ]
机构
[1] Tech Univ Denmark, Natl Food Inst, DK-2860 Saborg, Denmark
[2] Purdue Univ, Dept Food Sci, W Lafayette, IN 47907 USA
[3] Natl Vet Inst, SE-75189 Uppsala, Sweden
基金
美国农业部;
关键词
Food safety; Campylobacter; Zoonoses; Poultry; Pathogen; Rapid detection; REAL-TIME PCR; QUANTITATIVE RISK-ASSESSMENT; ETHIDIUM MONOAZIDE; RAPID DETECTION; THERMOPHILIC CAMPYLOBACTER; CHICKEN CARCASSES; REACTION ASSAY; MESSENGER-RNA; UNITED-STATES; JEJUNI;
D O I
10.1016/j.mimet.2015.03.007
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Improved monitoring tools are important for the control of Campylobacter bacteria in poultry production. Standardized reference culture methods issued by national and international standardization organizations are time-consuming, cumbersome and not amenable to automation for screening of large numbers of samples. The ultimate goal for rapid monitoring of Campylobacter is to prevent contaminated meat from entering the food market. Currently, real-time PCR is fulfilling abovementioned criteria to a certain extent. Further development of real-time PCR, microarray PCR, miniaturized biosensors, chromatographic techniques and DNA sequencing can improve our monitoring capacity at a lower cost. Combined with innovative sampling and sample treatment, these techniques could become realistic options for on-farm and liquid-sample monitoring at slaughterhouses. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:118 / 125
页数:8
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