Enantiomer separation by open-tubular liquid chromatography and electrochromatography in cellulose-coated capillaries

被引：92

作者：

Francotte, E

Jung, M

机构：

[1] Ciba, Pharmaceutical Research, K-122.P.25

来源：

CHROMATOGRAPHIA | 1996年 / 42卷 / 9-10期

关键词：

open-tubular liquid chromatography; capillary electrochromatography and electrophoresis; cellulose derivatives; chiral stationary phase (CSP); enantiomer separation;

D O I：

10.1007/BF02290286

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The feasibility of enantiomer separations by normal-phase and reversed-phase open-tubular liquid chromatography as well as by capillary electrochromatography in 50 mu m i.d. fused silica capillaries coated with cellulose derivatives is demonstrated for a number of chiral pharmaceuticals. The excellent enantioselectivity of 3,5-dimethylphenylcarbamoyl cellulose and para-methylbenzoyl cellulose has been transferred from HPLC to a miniaturized, open-tubular system. A comparison of HPLC, OTLC and CEC is made and the influence of retention and temperature on efficiency, selectivity and resolution is discussed.

引用

页码：521 / 527

页数：7

共 38 条

[1] DERIVATIZED CYCLODEXTRINS IMMOBILIZED ON FUSED-SILICA CAPILLARIES FOR ENANTIOMERIC SEPARATIONS VIA CAPILLARY ELECTROPHORESIS, GAS-CHROMATOGRAPHY, OR SUPERCRITICAL FLUID CHROMATOGRAPHY [J].