MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression

被引：12
作者：
Hu, Jiangwei [1 ]
Xiang, Xinqin [1 ]
Guan, Wei [1 ]
Lou, Weihua [1 ]
He, Junming [1 ]
Chen, Jian [1 ]
Fu, Yin [1 ]
Lou, Guoliang [1 ]
机构：
[1] Yiwu Cent Hosp, Dept Cardiovasc Surg, 699 Jiangdong Dong Lu, Yiwu City 322000, Zhejiang, Peoples R China
来源：
JOURNAL OF CARDIOTHORACIC SURGERY | 2021年 / 16卷 / 01期
关键词：
miR-497-5p; CDCA4; LUSC; Proliferation; Migration; Invasion; GROWTH; CANCER; FAMILY; E2F; METASTASIS; MIRNA;
D O I：
10.1186/s13019-021-01698-2
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Background So far, few have concerned miR-497-5p in lung squamous cell carcinoma (LUSC). Methods MiR-497-5p expression in LUSC was measured by qRT-PCR. Its impacts on tumor-related cell behaviors were investigated by CCK8 assay, scratch healing assay, flow cytometry and Transwell invasion methods. In addition, interaction between miR-497-5p and CDCA4 in LUSC was also elucidated through rescue experiment, western blot, dual-luciferase, and bioinformatics analysis. Results Low level of miR-497-5p was confirmed in LUSC tissue and cells. Overexpressed miR-497-5p markedly inhibited cancer progression. miR-497-5p restrained CDCA4 expression. Rescue assay showed that overexpressing miR-497-5p eliminated effect of overexpressed CDCA4. Conclusion By targeting CDCA4, miR-497-5p restrained development of LUSC.
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页数：8
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