Caged substrates for protein labeling and immobilization

被引：21

作者：

Banala, Sambashiva ^{[1
]}

Arnold, Anke ^{[1
]}

Johnsson, Kai ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Inst Chem Sci & Engn, CH-1015 Lausanne, Switzerland

来源：

CHEMBIOCHEM | 2008年 / 9卷 / 01期

关键词：

caged molecules; protein labeling; protein microarrays; protein-protein interactions; proteomics;

D O I：

10.1002/cbic.200700472

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spotlight on protein labeling. The labeling of fusion proteins of O 6-alkylguanine-DNA alkyltransferase (AGT or SNAP-tag) is controlled with both spatial and temporal resolution by cageing the substrate with the 1-(2-nitrophenyl)ethyl group. The substrates can be uncaged simply by illumination. The light-controlled dimerization and light-directed immobilization of SNAP-tag fusion proteins is shown. (Chemical Equation Presented) © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：38 / 41

页数：4

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