Comparison of chemopreventive effects of Vitamin E plus selenium versus melatonin in 7,12-dimethylbenz(a) anthracene-induced mouse brain damage

被引:14
作者
Batcioglu, K [1 ]
Karagözler, AA
Ozturk, IC
Genc, M
Bay, A
Ozturk, F
Aydogdu, N
机构
[1] Inonu Univ, Fac Pharm, Dept Biochem, Malatya, Turkey
[2] Adnan Menderes Univ, Fac Arts & Sci, Dept Chem, Aydin, Turkey
[3] Inonu Univ, Fac Pharm, Dept Publ Hlth, Malatya, Turkey
[4] Inonu Univ, Fac Pharm, Dept Histol, Malatya, Turkey
[5] Trakya Univ, Fac Pharm, Dept Physiol, Edirne, Turkey
来源
CANCER DETECTION AND PREVENTION | 2004年 / 29卷 / 01期
关键词
chemical carcinogenesis; 7,12-DMBA; free radicals; oxidative damage; melatonin; Vitamin E; selenium; brain;
D O I
10.1016/j.cdp.2004.06.006
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
In this work, the protective effect of Vitamin E plus selenium (Vit E + Se) and melatonin against 7,12-dimethylbenz(a)anthracene (7,12DMBA)-induced changes in superoxide dismutase (SOD), glutathione peroxidase (GSHPx), catalase (CAT) and carbonic anhydrase (CA) activities and malonedialdehyde (MDA) levels of mouse brain were compared. 12-month old mice were divided into four groups each including 10 animals. The first group served as control group. The second group was treated with 7,12-DMBA (20 mg/(kg day)). The third group was treated with 7,12-DMBA and Vitamin E (90 mu g/(individual day)) and selenium (1.8 mu g/(individual day)) simultaneously. The fourth group was treated with 7, 12-DMBA and melatonin (4.2 mg/(kg day)) simultaneously. Treatment continued for 21 days after which the mice were sacrificed and brain homogenates were prepared. 7,12-DMBA treated group exhibited significantly decreased levels of brain SOD, GSHPx, CAT and CA activities and increased MDA levels as compared to control. Vitamin E + Se fully or partially restored enzyme inhibition except for SOD. Lipid peroxidation was also reduced in Vitamin E + Se treated group. Melatonin provided a better protection for SOD, GSHPx and CAT, and a plausible protection for CA activity. Protection against lipid peroxidation measured as MDA in melatonin treated group was appreciable although slightly lesser than the protection provided by Vitamin E + Se. The results imply that Vitamin E + Se and melatonin both provide chemoprevention against 7,12-DMBA-induced oxidative stress in mouse brain. (c) 2004 International Society for Preventive Oncology. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:54 / 58
页数:5
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