Plant tissue-based chemiluminescence flow biosensor for glycolic acid

被引：53

作者：

Li, BX ^{[1
]}

Zhang, ZJ ^{[1
]}

Jin, Y ^{[1
]}

机构：

[1] Shaanxi Normal Univ, Dept Chem, Xian 710062, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2001年 / 73卷 / 06期

关键词：

D O I：

10.1021/ac000796k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel plant tissue-based chemiluminescence (CL) biosensor for glycolic acid combined with now injection analysis is proposed in this paper. The spinach tissue acts as the molecular recognition element. Glycolic acid is oxidized by oxygen under the catalysis of glycolate oxidase in the tissue column to produce hydrogen peroxide, which can react with luminol in the presence of peroxidase of spinach tissue to generate a CL signal. The CL emission intensity was linear with glycolic acid concentration in the range of 4 x 10(-3)-4 x 10(-6) mol/L and the detection limit was 1.3 x 10(-6) mol/L. The biosensor was stable for about 3 weeks. A complete analysis, including sampling and washing, could be performed in 1.5 min with a relative standard deviation of 1.7%.

引用

页码：1203 / 1206

页数：4

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