Myc stimulates cell cycle progression through the activation of Cdk1 and phosphorylation of p27

被引：57

作者：

Garcia-Gutierrez, Lucia ^{[1
,2
,7
]}

Bretones, Gabriel ^{[1
,2
,8
]}

Molina, Ester ^{[1
,2
]}

Arechaga, Ignacio ^{[1
,2
]}

Symonds, Catherine ^{[4
,9
]}

Acosta, Juan C. ^{[3
]}

Blanco, Rosa ^{[1
,2
]}

Fernandez, Adrian ^{[1
,2
]}

Alonso, Leticia ^{[1
,2
]}

Sicinski, Piotr ^{[5
]}

Barbacid, Mariano ^{[4
]}

Santamaria, David ^{[6
]}

Leon, Javier ^{[1
,2
]}

机构：

[1] Univ Cantabria, Inst Biomed & Biotecnol Cantabria IBBTEC, CSIC, Santander, Spain

[2] Univ Cantabria, Dept Biol Mol, Santander, Spain

[3] Univ Edinburgh, Inst Genet & Mol Med, Edinburgh Canc Res UK Ctr, Edinburgh, Midlothian, Scotland

[4] CNIO, Expt Oncol, Mol Oncol Programme, Madrid, Spain

[5] Dana Farber Canc Inst, Dept Canc Biol, Boston, MA 02115 USA

[6] Univ Bordeaux, INSERM, U1218, ACTION Lab,IECB, Pessac, France

[7] Univ Coll Dublin, Syst Biol Ireland, Dublin, Ireland

[8] Univ Oviedo, Inst Univ Oncol IUOPA, Dept Bioquim & Biol Mol, E-33006 Oviedo, Spain

[9] EMD Serono, Global Oncol Franchise, Rockland, MA USA

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

关键词：

DEPENDENT KINASE INHIBITOR; C-MYC; P27(KIP1); EXPRESSION; CANCER; SEQUESTRATION; PROLIFERATION; REQUIREMENT; SUPPRESSION; FAMILY;

D O I：

10.1038/s41598-019-54917-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cell cycle stimulation is a major transforming mechanism of Myc oncoprotein. This is achieved through at least three concomitant mechanisms: upregulation of cyclins and Cdks, downregulation of the Cdk inhibitors p15 and p21 and the degradation of p27. The Myc-p27 antagonism has been shown to be relevant in human cancer. To be degraded, p27 must be phosphorylated at Thr-187 to be recognized by Skp2, a component of the ubiquitination complex. We previously described that Myc induces Skp2 expression. Here we show that not only Cdk2 but Cdk1 phosphorylates p27 at the Thr-187. Moreover, Myc induced p27 degradation in murine fibroblasts through Cdk1 activation, which was achieved by Myc-dependent cyclin A and B induction. In the absence of Cdk2, p27 phosphorylation at Thr-187 was mainly carried out by cyclin A2-Cdk1 and cyclin B1-Cdk1. We also show that Cdk1 inhibition was enough for the synthetic lethal interaction with Myc. This result is relevant because Cdk1 is the only Cdk strictly required for cell cycle and the reported synthetic lethal interaction between Cdk1 and Myc.

引用

页数：17