Effect of CEACAM-1 knockdown in human colorectal cancer cells

被引：18
作者：
Han, Zhong-Min [1 ]
Huang, He-Mei [1 ]
Sun, Yong-Wu [1 ]
机构：
[1] Zhengzhou Railway Vocat & Tech Coll, Dept Med Technol, 9 Qiancheng Rd, Zhengzhou 450052, Henan, Peoples R China
来源：
ONCOLOGY LETTERS | 2018年 / 16卷 / 02期
关键词：
colon cancer; carcinoembryonic antigen-related cell adhesion molecule 1; HCT-8 cell line; small interfering RNA; ADHESION MOLECULE-1; BILIARY GLYCOPROTEIN; PANCREATIC-CANCER; EXPRESSION; METASTASIS; FAMILY; MEMBER; GENE;
D O I：
10.3892/ol.2018.8835
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM-1) is the major antigen of the CD66 cluster of granulocyte differentiation antigens. The present study aimed to assess the biological function of CEACAM-1 on the growth of human colorectal cancer (CRC) cells in vitro. Treatment of cultured CRC HCT-8 cells with CEACAM-1-specific siRNA successfully downregulated CEACAM-1 expression by 61% compared with control cells. The effects of CEACAM-1 downregulation on HCT-8 cell proliferation and apoptosis were then assessed via Cell Counting kit-8 assay and flow cytometry, respectively. The results demonstrated that siRNA-induced CEACAM-1 downregulation significantly inhibited proliferation and increased apoptosis, but had no significant effect on cell cycle progression in HCT-8 cells. Together, these results suggest that CEACAM-1 activity is critical to CRC growth, and thus, CEACAM-1 may be a promising therapeutic target for the treatment of CRC.
引用
收藏
页码：1622 / 1626
页数：5
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