New amino and acetamido monomethine cyanine dyes for the detection of DNA in agarose gels

被引：17
作者：
El-Shishtawy, Reda M.
Santos, Cecilia R.
Goncalves, Isabel
Marcelino, Helena
Almeida, Paulo [1 ]
机构：
[1] Univ Beira Interior, Dept Quim, P-6201001 Covilha, Portugal
[2] Univ Beira Interior, Unidade I&D Mat Text & Papeleiros, P-6201001 Covilha, Portugal
[3] Univ Beira Interior, CICS, P-6201001 Covilha, Portugal
来源：
BIOORGANIC & MEDICINAL CHEMISTRY | 2007年 / 15卷 / 16期
关键词：
monomethine cyanine dyes; ethidium bromide; DNA; gel electrophoresis;
D O I：
10.1016/j.bmc.2007.05.043
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Some new monomethine cyanine dyes derived from quinoline and benzothiazole have been prepared and characterized by H-1 and C-13 NMR, FTIR, FABHRMS, and visible spectroscopy. The dyes containing amino and acetamido groups were conveniently synthesized by the condensation of two p-toluenesulfonate heterocyclic quaternary salts and were obtained in the forms of iodide, bromide, and tosylate counteranions. These dyes were compared to ethidium bromide as stains for DNA in electrophoretic gels. The overall results obtained for the sensitivity of these dyes suggest the suitability of acetamido moiety over the amine one and bromide as the counteranion when compared with iodide and tosylate, with a similar capacity of DNA detection in relation to the ethidium bromide stain over the concentration range of 1-3 ng. (c) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码：5537 / 5542
页数：6
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