Recovery from DNA replicational stress is the essential function of the S-phase checkpoint pathway

被引：388

作者：

Desany, BA ^{[1
]}

Alcasabas, AA ^{[1
]}

Bachant, JB ^{[1
]}

Elledge, SJ ^{[1
]}

机构：

[1] Baylor Coll Med, Howard Hughes Med Inst, Verna & Marrs McLean Dept Biochem, Houston, TX 77030 USA

来源：

GENES & DEVELOPMENT | 1998年 / 12卷 / 18期

关键词：

DNA replication; S-phase; checkpoint pathway; ribonucleotide reductase; nucleotide levels;

D O I：

10.1101/gad.12.18.2956

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

RAD53 and MEC1 are essential genes required for the transcriptional and cell cycle responses to DNA damage and DNA replication blocks. We have examined the essential function of these genes and found that their lethality but not their checkpoint defects can be suppressed by increased expression of genes encoding ribonucleotide reductase. Analysis of viable null alleles revealed that Mec1 plays a greater role in response to inhibition of DNA synthesis than Rad53. The loss of survival in mec1 and rad53 null or point mutants in response to transient inhibition of DNA synthesis is not a result of inappropriate anaphase entry but primarily to an inability to complete chromosome replication. We propose that this checkpoint pathway plays an important role in the maintenance of DNA synthetic capabilities when DNR replication is stressed.

引用

页码：2956 / 2970

页数：15

共 48 条

[1] IDENTIFICATION AND CHARACTERIZATION OF NEW ELEMENTS INVOLVED IN CHECKPOINT AND FEEDBACK CONTROLS IN FISSION YEAST [J].