Isolation and characterization of microsatellites in Chinese soft-shelled turtle, Pelodiscus sinensis

被引:16
作者
Que, Yanfu
Zhu, Bin
Rosenthal, H.
Chang, Jianbo
机构
[1] Chinese Acad Sci, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Wuhan 430079, Peoples R China
[2] Minist Water Resources, Inst Hydroecol, Wuhan 430072, Peoples R China
[3] Chinese Acad Sci, Wuhan 430072, Peoples R China
[4] World Sturgeon Conservat Soc, D-21629 Neu Wulmstorf, Germany
[5] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
来源
MOLECULAR ECOLOGY NOTES | 2007年 / 7卷 / 06期
关键词
microsatellites; Pelodiscus sinensis; polymorphism;
D O I
10.1111/j.1471-8286.2007.01850.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fifteen polymorphic microsatellite loci were developed for the Chinese soft-shelled turtle (Pelodiscus sinensis) from the (GT)(n) microsatellite-enriched genomic library, using the fast isolation by amplified fragment length polymorphism of sequences containing repeats protocol. The polymorphism of all 15 loci ranged from two to seven alleles with observed heterozygosities ranging from 0.03 to 0.98 (mean 0.43) in one population of 40 individuals. These novel loci will be helpful for understanding the population structure at genetic level and marker-assisted breeding of this vulnerable species.
引用
收藏
页码:1265 / 1267
页数:3
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