Location of the O-methyl groups in the O polysaccharide of Pseudomonas syringae pv. phaseolicola

被引：20
|
作者：
Zdorovenko, EL
Ovod, VV
Zatonsky, GV
Shashkov, AS
Kocharova, NA
Knirel, YA
机构：
[1] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 117913, Russia
[2] Univ Tampa, Inst Med Technol, Tampere, Finland
来源：
CARBOHYDRATE RESEARCH | 2001年 / 330卷 / 04期
关键词：
lipopolysaccharides; O-polysaccharides; O-methylation; phytopathogens; Pseudomonas syringae;
D O I：
10.1016/S0008-6215(01)00005-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The O-methylation pattern of the O polysaccharide (OPS) of the lipopolysaccharide of Pseudomonas syringae pv. phaseolicola GSPB 1552 was revealed by methylation (CD3I) analysis, Smith degradation, and NMR spectroscopy. Together with the major O repeats consisting of D-rhamnopyranose (D-Rhap) and D-fucofuranose (D-Fucf), there are minor repeats (similar to 30%) containing 3-O-methyl-D-rhamnose (D-acofriose), which is 2-substituted in the interior repeats and occupies the terminal non-reducing end of the OPS. It was suggested that the methylated O repeats are linked to each other nearby the non-reducing end of the OPS and that the 'biological' O repeat of the OPS has the following structure: [GRAPHICS] (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：505 / 510
页数：6
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