Silencing PLOD2 attenuates cancer stem cell-like characteristics and cisplatin-resistant through Integrin β1 in laryngeal cancer

被引:7
作者
Song, Meiyan [1 ]
Liu, Xing [2 ]
Li, Tao [3 ]
Zhang, Yueqin [4 ]
Zhao, Xiaoyan [4 ]
Sun, Wen [3 ]
Li, Zhen [4 ]
机构
[1] Yantaishan Hosp, Yantai, Shandong, Peoples R China
[2] Qingdao Hiser Hosp, Qingdao Hosp Tradit Chinese Med, Dept Otolaryngol, Qingdao, Shandong, Peoples R China
[3] Zibo Municipal Hosp, Dept Otolaryngol, Zibo, Shandong, Peoples R China
[4] Yantaishan Hosp, Dept Otolaryngol, 10087 Keji Ave,Laishan Dist, Yantai 264000, Shandong, Peoples R China
来源
TRANSLATIONAL ONCOLOGY | 2022年 / 22卷
关键词
PLOD2; Integrin?1; Laryngeal cancer; Drug-resistance; Cancer stem cells;
D O I
10.1016/j.tranon.2022.101460
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Laryngeal cancer (LC) is an aggressive malignancy resistant to drug treatments. It has been postulated that cancer stem cells (CSCs) persist in a unique population of cancer cells involved in tumor progression and drug resistance. In the present study, the effects of PLOD2 expression on ordinary and Cisplatin (DDP)-resistance (R) cells were investigated in TU686 and TU138 cells and Xenograft model. Cell viability, invasion and cell apoptosis, CD44 and CD133 expressions, MRP1 and P-gp expressions were measured by CCK-8 assay, Transwell, flow cytometry, immunofluorescence and Western blotting respectively. The results of our study demonstrated that suppressing the expression of PLOD2 could meditate LC stem cell-like features by decrease cell viability and invasion, increase apoptotic rate, decrease CD44 and CD133 expressions via Integrin beta 1. Meanwhile, the inhibition of PLOD2 expression could decrease P-gp and MRP1expression thus markedly regulate DDP-R LC cells stemness and drug-resistance via Integrin beta 1. Our findings provided a new rationale for subsequent academic and clinical research on LC drug-resistance.
引用
收藏
页数:12
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共 36 条
  • [11] PD-L1 interacts with Frizzled 6 to activate β-catenin and form a positive feedback loop to promote cancer stem cell expansion
    Fu, Lingchen
    Fan, Jia
    Maity, Sudipa
    McFadden, Grant
    Shi, Yixin
    Kong, Wei
    [J]. ONCOGENE, 2022, 41 (08) : 1100 - 1113
  • [12] Cancer stem cells in laryngeal cancer: what we know
    Greco, A.
    Rizzo, Maria Ida
    De Virgilio, A.
    Gallo, A.
    Fusconi, M.
    Pagliuca, G.
    Martellucci, S.
    Turchetta, R.
    De Vincentiis, M.
    [J]. EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY, 2016, 273 (11) : 3487 - 3495
  • [13] García-León FJ, 2017, ACTA OTORRINOLAR ESP, V68, P212, DOI 10.1016/j.otorri.2016.11.005
  • [14] HEAD AND NECK SQUAMOUS CELL CARCINOMA
    不详
    [J]. NATURE REVIEWS DISEASE PRIMERS, 2020, 6 (01):
  • [15] Natural products as multidrug resistance modulators in cancer
    Kumar, Amit
    Jaitak, Vikas
    [J]. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2019, 176 : 268 - 291
  • [16] Cancer Stem Cell Traits in Tumor Spheres Derived from Primary Laryngeal Carcinoma Cell Lines
    Kumbar, Vijay Mahadev
    Muddapur, Uday M.
    Bhat, Kishore G.
    Shwetha, H. R.
    Kugaji, Manohar S.
    Peram, Malleswara Rao
    Dindawar, Santosh
    [J]. CONTEMPORARY CLINICAL DENTISTRY, 2021, 12 (03) : 247 - 254
  • [17] Cancer Stem Cells and Neovascularization
    Li, Fengkai
    Xu, Jiahui
    Liu, Suling
    [J]. CELLS, 2021, 10 (05)
  • [18] MRP1 and its role in anticancer drug resistance
    Lu, Jamie F.
    Pokharel, Deep
    Bebawy, Mary
    [J]. DRUG METABOLISM REVIEWS, 2015, 47 (04) : 406 - 419
  • [19] The Probable Role of Tumor Stem Cells for Lymph Node Metastasis in Supraglottic Carcinoma
    Lu, Sumei
    Tian, Jiajun
    Lv, Zhenghua
    Wang, Haibo
    Bai, Xiaohui
    Liu, Wenwen
    Li, Jianfeng
    Xu, Wei
    [J]. PATHOLOGY & ONCOLOGY RESEARCH, 2011, 17 (01) : 33 - 38
  • [20] Mechanisms of integrin activation and trafficking
    Margadant, Coert
    Monsuur, Hanneke N.
    Norman, Jim C.
    Sonnenberg, Arnoud
    [J]. CURRENT OPINION IN CELL BIOLOGY, 2011, 23 (05) : 607 - 614