Homo-catiomer integration into PEGylated polyplex micelle from block-catiomer for systemic anti-angiogenic gene therapy for fibrotic pancreatic tumors

被引：57

作者：

Chen, Qixian ^{[1
]}

Osada, Kensuke ^{[1
]}

Ishii, Takehiko ^{[5
]}

Oba, Makoto ^{[2
]}

Uchida, Satoshi ^{[4
]}

Tockary, Theofilus A. ^{[1
]}

Endo, Taisuke ^{[1
]}

Ge, Zhishen ^{[1
]}

Kinoh, Hiroaki ^{[1
]}

Kano, Mitsunobu R. ^{[3
]}

Itaka, Keiji

Kataoka, Kazunori ^{[1
,4
,5
]}

机构：

[1] Univ Tokyo, Grad Sch Engn, Dept Mat Engn, Bunkyo Ku, Tokyo 1138656, Japan

[2] Univ Tokyo, Grad Sch Engn, Dept Clin Vasc Regenerat, Bunkyo Ku, Tokyo 1138655, Japan

[3] Univ Tokyo, Grad Sch Engn, Dept Mol Pathol, Bunkyo Ku, Tokyo 1138655, Japan

[4] Univ Tokyo, Grad Sch Engn, Dept Mat Engn, Bunkyo Ku, Tokyo 1130033, Japan

[5] Univ Tokyo, Grad Sch Engn, Dept Bioengn, Tokyo 1130033, Japan

来源：

BIOMATERIALS | 2012年 / 33卷 / 18期

基金：

日本学术振兴会;

关键词：

DNA; Micelle; Nanoparticle; Gene transfer; In vitro test; In vivo test; POLYMERIC MICELLES; CANCER; COPOLYMERS; TRANSFECTION; VECTORS; DRUG;

D O I：

10.1016/j.biomaterials.2012.03.017

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Homo-poly(N'-[N-(2-aminoethyl)-2-aminoehtyljaspartamide) [PAsp(DET), HI was attempted to integrate into poly (ethylene glycol) (PEG)-b-PAsp(DET)] (B) formulated polyplex micelle with the aim of enhancing cell transfection efficiency for PEGylated polyplex micelle via H integration. In vitro evaluations verified H integration of potent stimulation in enhancing cell-transfecting activity of PEGylated polyplex micelles via promoted cellular uptake and facilitated endosome escape. In vivo anti-angiogenic tumor suppression evaluations validated the feasibility of H integration in promoting gene transfection to the affected cells via systemic administration, where loaded anti-angiogenic gene remarkably expressed in the tumor site, thereby imparting significant inhibitory effect on the growth of vascular endothelial cells, ultimately leading to potent tumor growth suppression. These results demonstrated potency of H integration for enhanced transfection activity and potential usage in systemic applications, which could have important implications on the strategic use of H integration in the non-viral gene carrier design. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：4722 / 4730

页数：9

共 34 条

[1]

Cabral H, 2011, NAT NANOTECHNOL, V6, P815, DOI [10.1038/nnano.2011.166, 10.1038/NNANO.2011.166]

[2] Angiogenesis in cancer and other diseases [J].

Carmeliet, P ;

Jain, RK .

NATURE, 2000, 407 (6801) :249-257

[3]

FOLKMAN J, 1971, NEW ENGL J MED, V285, P1182

[4]

Freelove R, 2006, AM FAM PHYSICIAN, V73, P485

[5] FORMATION OF POLYION COMPLEX MICELLES IN AN AQUEOUS MILIEU FROM A PAIR OF OPPOSITELY-CHARGED BLOCK-COPOLYMERS WITH POLY(ETHYLENE GLYCOL) SEGMENTS [J].

HARADA, A ;

KATAOKA, K .

MACROMOLECULES, 1995, 28 (15) :5294-5299

[6] Chemoradiotherapy in the Management of Locally Advanced Pancreatic Carcinoma: A Qualitative Systematic Review [J].

Huguet, Florence ;

Girard, Nicolas ;

Seblain-El Guerche, Clotilde ;

Hennequin, Christophe ;

Mornex, Francoise ;

Azria, David .

JOURNAL OF CLINICAL ONCOLOGY, 2009, 27 (13) :2269-2277

[7] Biodegradable polyamino acid-based polycations as safe and effective gene carrier minimizing cumulative toxicity [J].

Itaka, Keiji ;

Ishii, Takehiko ;

Hasegawa, Yoko ;

Kataoka, Kazunori .

BIOMATERIALS, 2010, 31 (13) :3707-3714

[8] Polymeric micelles - a new generation of colloidal drug carriers [J].

Jones, MC ;

Leroux, JC .

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 1999, 48 (02) :101-111

[9] A PEG-based biocompatible block catiomer with high buffering capacity for the construction of polyplex micelles showing efficient gene transfer toward primary cells [J].

Kanayama, Naoki ;

Fukushima, Shigeto ;

Nishiyama, Nobuhiro ;

Itaka, Keiji ;

Jang, Woo-Dong ;

Miyata, Kanjiro ;

Yamasaki, Yuichi ;

Chung, Ung-il ;

Kataoka, Kazunori .

CHEMMEDCHEM, 2006, 1 (04) :439-444

[10] Introduction of stearoyl moieties into a biocompatible cationic polyaspartamide derivative, PAsp(DET), with endosomal escaping function for enhanced siRNA-mediated gene knockdown [J].

Kim, Hyun Jin ;

Ishii, Atsushi ;

Miyata, Kanjiro ;

Lee, Yan ;

Wu, Shourong ;

Oba, Makoto ;

Nishiyama, Nobuhiro ;

Kataoka, Kazunori .

JOURNAL OF CONTROLLED RELEASE, 2010, 145 (02) :141-148

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