Down-regulation of an Auxin Response Factor in the tomato induces modification of fine pectin structure and tissue architecture

被引:53
作者
Guillon, Fabienne [1 ]
Philippe, Sully [1 ]
Bouchet, Brigitte [1 ]
Devaux, Marie-Francoise [1 ]
Frasse, Pierre [2 ]
Jones, Brian [2 ]
Bouzayen, Mondher [2 ]
Lahaye, Marc [1 ]
机构
[1] INRA, UR Biopolymeres Interact Assemblages 1268, F-44300 Nantes, France
[2] INRA, INTP ENSA, UMR Genom & Biotechnol Fruits 990, F-31326 Castanet Tolosan, France
关键词
Auxin; cell wall; firmness; fruit; pectin; pericarp; tomato;
D O I
10.1093/jxb/erm323
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
It has previously been shown that down-regulation of an auxin response factor gene (DR12) results in pleiotropic phenotypes including enhanced fruit firmness in antisense transgenic tomato (AS-DR12). To uncover the nature of the ripening-associated modifications affecting fruit texture, comparative analyses were performed of pectin composition and structure in cell wall pericarp tissue of wild-type and AS-DR12 fruit at mature green (MG) and red-ripe (RR) stages. Throughout ripening, pectin showed a decrease in methyl esterification and in the content of galactan side chains in both genotypes. At mature green stage, pectin content in methyl ester groups was slightly higher in AS-DR12 fruit than in wild type, but this ratio was reversed at the red-ripe stage. The amount of water- and oxalate-soluble pectins increased at the red-ripe stage in the wild type, but decreased in AS-DR12. The distribution of methyl ester groups on the homogalaturonan backbone differed between the two genotypes. There was no evidence of more calcium cross-linked homogalacturan involved in cell-to-cell adhesion in AS-DR12 compared with wild-type fruit. Furthermore, the outer pericarp contains higher proportion of small cells in AS-DR12 fruit than in wild type and higher occurrence of (1 -> 5) alpha-L-arabinan epitope at the RR stage. It is concluded that the increased firmness of transgenic fruit does not result from a major impairment of ripening-related pectin metabolism, but rather involves differences in pectin fine structure associated with changes in tissue architecture.
引用
收藏
页码:273 / 288
页数:16
相关论文
共 80 条
  • [1] Micromechanical and geometrical properties of tomato skin related to differences in puncture injury susceptibility
    Allende, A
    Desmet, M
    Vanstreels, E
    Verlinden, BE
    Nicolaï, BM
    [J]. POSTHARVEST BIOLOGY AND TECHNOLOGY, 2004, 34 (02) : 131 - 141
  • [2] Characterisation of pectin subunits released by an optimised combination of enzymes
    Bonnin, E
    Dolo, E
    Le Goff, A
    Thibault, JF
    [J]. CARBOHYDRATE RESEARCH, 2002, 337 (18) : 1687 - 1696
  • [3] THE PREPARATION AND SUSCEPTIBILITY TO HYDROLYSIS OF NOVEL O-GALACTURONOYL DERIVATIVES OF CARBOHYDRATES
    BROWN, JA
    FRY, SC
    [J]. CARBOHYDRATE RESEARCH, 1993, 240 : 95 - 106
  • [4] Molecular aspects of cell wall modifications during fruit ripening
    Brownleader, MD
    Jackson, P
    Mobasheri, A
    Pantelides, AT
    Sumar, S
    Trevan, M
    Dey, PM
    [J]. CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION, 1999, 39 (02) : 149 - 164
  • [5] Cell wall disassembly in ripening fruit
    Brummell, DA
    [J]. FUNCTIONAL PLANT BIOLOGY, 2006, 33 (02) : 103 - 119
  • [6] Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants
    Brummell, DA
    Harpster, MH
    [J]. PLANT MOLECULAR BIOLOGY, 2001, 47 (1-2) : 311 - 340
  • [7] Pectic epitopes are differentially distributed in the cell walls of potato (Solanum tuberosum) tubers
    Bush, MS
    McCann, MC
    [J]. PHYSIOLOGIA PLANTARUM, 1999, 107 (02) : 201 - 213
  • [8] CHAIB J, 2007, IN PRESS J EXPT BOT, V58
  • [9] Cell expansion and endoreduplication show a large genetic variability in pericarp and contribute strongly to tomato fruit growth
    Cheniclet, C
    Rong, WY
    Causse, M
    Frangne, N
    Bolling, L
    Carde, JP
    Renaudin, JP
    [J]. PLANT PHYSIOLOGY, 2005, 139 (04) : 1984 - 1994
  • [10] Synthetic methyl hexagalacturonate hapten inhibitors of antihomogalacturonan monoclonal antibodies LM7, JIM5 and JIM7
    Clausen, MH
    Willats, WGT
    Knox, JP
    [J]. CARBOHYDRATE RESEARCH, 2003, 338 (17) : 1797 - 1800