Crucial role of the influenza virus NS2 (NEP) C-terminal domain in M1 binding and nuclear export of vRNP

被引：65

作者：

Shimizu, Teppei ^{[1
]}

Takizawa, Naoki ^{[1
,2
]}

Watanabe, Ken ^{[1
]}

Nagata, Kyosuke ^{[3
]}

Kobayashi, Nobuyuki ^{[1
,4
]}

机构：

[1] Nagasaki Univ, Grad Sch Biomed Sci, Lab Mol Biol Infect Agents, Nagasaki 8528521, Japan

[2] Nagasaki Univ, Inst Trop Med, Nagasaki 8528523, Japan

[3] Univ Tsukuba, Grad Sch Comprehens Human Sci, Dept Infect Biol, Tsukuba, Ibaraki 3058575, Japan

[4] AVSS Corp, Cent Res Ctr, Nagasaki 8518137, Japan

来源：

FEBS LETTERS | 2011年 / 585卷 / 01期

关键词：

Influenza virus; Nuclear export; NS2; M1; vRNP; MATRIX PROTEIN; RIBONUCLEOPROTEINS; TRANSPORT; IDENTIFICATION; ASSOCIATION; SIGNAL; CRM1; RNA;

D O I：

10.1016/j.febslet.2010.11.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The influenza virus genome replicates in the host cell nucleus, and the progeny viral ribonucleoproteins (vRNPs) are exported to the cytoplasm prior to maturation. The influenza virus NS2 protein has a nuclear export signal (NES) and binds to M1. It is therefore postulated that vRNP is exported from the nucleus by binding to NS2 through M1. However, the significance of the association between NS2 and M1 for the nuclear export of vRNP is still poorly understood. We herein demonstrate that the C-terminal domain of NS2 (residues 81-100) is essential for M1 binding and the nuclear export of progeny vRNPs. Structured summary: MINT-8057301, MINT-8057317:NS2 (uniprotkb:P03508) binds (MI:0407) to M1 (uniprotkb:P03485) by pull down (MI:0096) (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：41 / 46

页数：6

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