Interphase Fluorescence In Situ Hybridization on Selected Plasma Cells is Superior in the Detection of Cytogenetic Aberrations in Plasma Cell Dyscrasia

被引：11

作者：

Put, Natalie

Lemmens, Heidi

Wlodarska, Iwona

Konings, Peter ^{[2
]}

Moreau, Yves ^{[2
]}

Hagemeijer, Anne

Vandenberghe, Peter

Michaux, Lucienne ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Dept Human Genet, Ctr Menselijke Erfelijkheid, B-3000 Louvain, Belgium

[2] Katholieke Univ Leuven, Dept Elektrotech, B-3000 Louvain, Belgium

来源：

GENES CHROMOSOMES & CANCER | 2010年 / 49卷 / 11期

关键词：

MULTIPLE-MYELOMA; AMYLOIDOSIS; DIAGNOSIS; PROGNOSIS; LEUKEMIA;

D O I：

10.1002/gcc.20809

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Interphase fluorescence in situ hybridization (FISH) detects nonrandom cytogenetic abnormalities in plasma cell (PC) dyscrasia according to PC burden. However, when performed on cultured whole bone marrow (BM), it often fails to detect these aberrations. We have compared this interphase FISH technique with FISH after PC purification or identification to detect recurrent aberrations. In this study, 235 BM samples were collected from patients with multiple myeloma (MM) or related PC disorders regardless of disease status. All samples were analyzed in parallel. Clonal abnormalities were detected in 34.9% of cultured samples compared with 71.0% PC selected samples (P < 0.001). Moreover, FISH on PCs allowed to detect more abnormalities per case (P < 0.001) and identified higher percentages of abnormal nuclei (P < 0.001). This study indicates that FISH on PCs is the preferred technique for routine cytogenetic investigation of MM. (C) 2010 Wiley-Liss, Inc.

引用

页码：991 / 997

页数：7

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