Simultaneous Determination of 20 Pharmacologically Active Substances in Cow's Milk, Goat's Milk, and Human Breast Milk by Gas Chromatography-Mass Spectrometry

被引:78
作者
Azzouz, Abdelmonaim [1 ]
Jurado-Sanchez, Beatriz [2 ]
Souhail, Badredine [3 ]
Ballesteros, Evaristo [1 ]
机构
[1] Univ Jaen, EPS Linares, Dept Phys & Analyt Chem, Jaen 23700, Spain
[2] Univ Cordoba, Dept Analyt Chem, E-14071 Cordoba, Spain
[3] Univ Abdelmalek Essaadi, Fac Sci, Dept Chem, Tetouan 93002, Morocco
关键词
pharmacologically active substances; milk; continuous solid-phase extraction; gas chromatography-mass spectrometry; SOLID-PHASE EXTRACTION; PERFORMANCE LIQUID-CHROMATOGRAPHY; BOVINE-MILK; CHLORAMPHENICOL; PLASMA; DRUGS; LIVER; MEAT; THIAMPHENICOL; CARBAMAZEPINE;
D O I
10.1021/jf200364w
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
This paper reports a systematic approach to the development of a method that combines continuous solid-phase extraction and gas chromatography-mass spectrometry for the simultaneous determination of 20 pharmacologically active substances including antibacterials (chloramphenicol, florfenicol, pyrimethamine, thiamphenicol), nonsteroideal anti-inflammatories (diclofenac, flunixin, ibuprofen, ketoprofen, naproxen, mefenamic acid, niflumic acid, phenylbutazone), antiseptic (triclosan), antiepileptic (carbamazepine), lipid regulator (clofibric add), beta-blockers (metoprolol, propranolol), and hormones (17 alpha-ethinylestradiol, estrone, 17 beta-estradiol) in milk samples. The sample preparation procedure involves deproteination of the milk, followed by sample enrichment and cleanup by continuous solid-phase extraction. The proposed method provides a linear response over the range of 0.6-5000 ng/kg and features limits of detection from 0.2 to 1.2 ng/kg depending on the particular analyte. The method was successfully applied to the determination of pharmacologically active substance residues in food samples including whole, raw, half-skim, skim, and powdered milk from different sources (cow, goat, and human breast).
引用
收藏
页码:5125 / 5132
页数:8
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