Infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging analysis of biospecimens

被引：58

作者：

Bokhart, M. T. ^{[1
]}

Muddiman, D. C. ^{[1
]}

机构：

[1] North Carolina State Univ, Dept Chem, WM Keck FTMS Lab Human Hlth Res, Raleigh, NC 27695 USA

来源：

ANALYST | 2016年 / 141卷 / 18期

基金：

美国国家卫生研究院;

关键词：

IR-MALDESI MSI; ATMOSPHERIC-PRESSURE; TISSUE-SECTIONS; PROTEINS; MACROMOLECULES; BIOMOLECULES; ACQUISITION; PROTEOMICS; PEPTIDES; INTACT;

D O I：

10.1039/c6an01189f

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) is a technique well suited for analysis of biological specimens. This tutorial review focuses on recent advancements and applications of IR-MALDESI MSI to better understand key biological questions. Through optimization of user-defined source parameters, comprehensive and quantitative MSI data can be obtained for a variety of analytes. The effect of an ice matrix layer is well defined in the context of desorption dynamics and resulting ion abundance. Optimized parameters and careful control of conditions affords quantitative MSI data which provides valuable information for targeted, label-free drug distribution studies and untargeted metabolomic datasets. Challenges and limitations of MSI using IR-MALDESI are addressed in the context of the bioimaging field.

引用

页码：5236 / 5245

页数：10

共 50 条

[1] Assessing drug and metabolite detection in liver tissue by UV-MALDI and IR-MALDESI mass spectrometry imaging coupled to FT-ICR MS [J].

Barry, Jeremy A. ;

Groseclose, M. Reid ;

Robichaud, Guillaume ;

Castellino, Stephen ;

Muddiman, David C. .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2015, 377 :448-455

[2] Mapping Antiretroviral Drugs in Tissue by IR-MALDESI MSI Coupled to the Q Exactive and Comparison with LC-MS/MS SRM Assay [J].

Barry, Jeremy A. ;

Robichaud, Guillaume ;

Bokhart, Mark T. ;

Thompson, Corbin ;

Sykes, Craig ;

Kashuba, Angela D. M. ;

Muddiman, David C. .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2014, 25 (12) :2038-2047

[3] Global optimization of the infrared matrix-assisted laser desorption electrospray ionization (IR MALDESI) source for mass spectrometry using statistical design of experiments [J].

Barry, Jeremy A. ;

Muddiman, David C. .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2011, 25 (23) :3527-3536

[4] Quantitative mass spectrometry imaging of emtricitabine in cervical tissue model using infrared matrix-assisted laser desorption electrospray ionization [J].

Bokhart, Mark T. ;

Rosen, Elias ;

Thompson, Corbin ;

Sykes, Craig ;

Kashuba, Angela D. M. ;

Muddiman, David C. .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2015, 407 (08) :2073-2084

[5] Mass spectrometry of intact neutral macromolecules using intense non-resonant femtosecond laser vaporization with electrospray post-ionization [J].

Brady, John J. ;

Judge, Elizabeth J. ;

Levis, Robert J. .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2009, 23 (19) :3151-3157

[6] Molecular imaging of biological samples: Localization of peptides and proteins using MALDI-TOF MS [J].

Caprioli, RM ;

Farmer, TB ;

Gile, J .

ANALYTICAL CHEMISTRY, 1997, 69 (23) :4751-4760

[7]

Castaing R., 1962, J MICROSCOPIE, V1, P395

[8] Analysis of trace fibers by IR-MALDESI imaging coupled with high resolving power MS [J].

Cochran, Kristin H. ;

Barry, Jeremy A. ;

Robichaud, Guillaume ;

Muddiman, David C. .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2015, 407 (03) :813-820

[9] Direct Analysis of Textile Fabrics and Dyes Using Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometry [J].

Cochran, Kristin H. ;

Barry, Jeremy A. ;

Muddiman, David C. ;

Hinks, David .

ANALYTICAL CHEMISTRY, 2013, 85 (02) :831-836

[10] MALDI mass spectrometric imaging meets "omics": recent advances in the fruitful marriage [J].

Crecelius, A. C. ;

Schubert, U. S. ;

von Eggeling, F. .

ANALYST, 2015, 140 (17) :5806-5820

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