The relationship between gender, knowledge of biotechnology, attitudes toward biotechnology, and various socio-demographic variables was investigated using the Eurobarometer 52.1. It was found that neither socio-demographics, nor differing levels of scientific knowledge could explain females' greater probability of being pessimistic toward biotechnology. After running separate models for males and females, it was discovered that, for males, more knowledge of biotechnology decreased their probability of being pessimistic about science, but for females more knowledge of biotechnology actually lead to a greater probability of being pessimistic. Further, a gender-education interaction was discovered that revealed that, for males, education and knowledge of biotechnology have independent effects on attitudes, but for females education has no effect on attitudes towards biotechnology when knowledge is controlled. The results for females complicate the deficit model of social support for science, which posits that more knowledge of science always leads to more positive attitudes.