Expression profiles of microRNAs after focal cerebral ischemia/reperfusion injury in rats

被引：31
作者：
Zhai, Fengguo [1 ]
Zhang, Xiuping [1 ]
Guan, Yue [2 ]
Yang, Xudong [1 ]
Li, Yang [3 ]
Song, Gaochen
Guan, Lixin [1 ,4 ]
机构：
[1] Mudanjiang Med Univ, Dept Pharmacol, Mudanjiang 157011, Heilongjiang Pr, Peoples R China
[2] Mudanjiang Med Univ, Dept Endocrinol, Mudanjiang 157011, Heilongjiang Pr, Peoples R China
[3] Mudanjiang Med Univ, Dept Thorac Surg, Mudanjiang 157011, Heilongjiang Pr, Peoples R China
[4] Mudanjiang Med Univ, Dept Biochem, Mudanjiang 157011, Heilongjiang Pr, Peoples R China
来源：
NEURAL REGENERATION RESEARCH | 2012年 / 7卷 / 12期
基金：
中国国家自然科学基金;
关键词：
focal cerebral ischemia; microRNA; apoptosis; bcl-2; caspase-3; neural regeneration; BCL-2; FAMILY-MEMBERS; ARTERY OCCLUSION; ANIMAL-MODEL; BRAIN-INJURY; CELL-DEATH; ISCHEMIA; APOPTOSIS; CASPASES; NEURONS; DISEASE;
D O I：
10.3969/j.issn.1673-5374.2012.12.007
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Rat models of focal cerebral ischemia/reperfusion injury were established by occlusion of the middle cerebral artery. Microarray analysis showed that 24 hours after cerebral ischemia, there were nine up-regulated and 27 down-regulated microRNA genes in cortical tissue. Bioinformatic analysis showed that bcl-2 was the target gene of microRNA-384-5p and microRNA-494, and caspase-3 was the target gene of microRNA-129, microRNA-320 and microRNA-326. Real-time PCR and western blot analyses showed that 24 hours after cerebral ischemia, bcl-2 mRNA and protein levels in brain tissue were significantly decreased, while caspase-3 mRNA and protein levels were significantly increased. This suggests that following cerebral ischemia, differentially expressed microRNA-384-5p, microRNA-494, microRNA-320, microRNA-129 and microRNA-326 can regulate bcl-2 and caspase-3 expression in brain tissue.
引用
收藏
页码：917 / 923
页数：7
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