&ITPolygonum aviculare L&IT. extract and quercetin attenuate contraction in airway smooth muscle

被引：30

作者：

Luo, Xi ^{[1
,2
]}

Xue, Lu ^{[1
,2
]}

Xu, Hao ^{[1
,2
]}

Zhao, Qing-Yang ^{[1
,2
]}

Wang, Qian ^{[1
,2
]}

She, Yu-Shan ^{[1
,2
]}

Zang, Dun-An ^{[1
,2
]}

Shen, Jinhua ^{[1
,2
]}

Peng, Yong-Bo ^{[1
,2
]}

Zhao, Ping ^{[1
,2
]}

Yu, Meng-Fei ^{[1
,2
]}

Chen, Weiwei ^{[1
,2
]}

Ma, Li-Qun ^{[1
,2
]}

Chen, Shu ^{[4
]}

Chen, Shanshan ^{[4
]}

Fu, Xiangning ^{[5
]}

Hu, Sheng ^{[6
]}

Nie, Xiaowei ^{[3
]}

Shen, Chenyou ^{[3
]}

Zou, Chunbin ^{[7
]}

Qin, Gangjian ^{[8
,9
]}

Dai, Jiapei ^{[10
]}

Ji, Guangju ^{[11
]}

Su, Yunchao ^{[12
]}

Hu, Shen ^{[13
]}

Chen, Jingyu ^{[3
]}

Liu, Qing-Hua ^{[1
,2
]}

机构：

[1] South Cent Univ Nationalities, Coll Life Sci, Inst Med Biol, Wuhan 430074, Hubei, Peoples R China

[2] South Cent Univ Nationalities, Coll Life Sci, Hubei Prov Key Lab Protect & Applicat Special Pla, Wuhan 430074, Hubei, Peoples R China

[3] Nanjing Med Univ, Wuxi Peoples Hosp, Jiangsu Key Lab Organ Transplantat, Dept Cardiothorac Surg,Lung Transplant Grp, Wuxi, Jiangsu, Peoples R China

[4] Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Cardiovasc Surg, Wuhan 430032, Hubei, Peoples R China

[5] Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Thorac, Wuhan 430032, Hubei, Peoples R China

[6] Hubei Canc Hosp, Dept Med Oncol, Wuhan 430079, Hubei, Peoples R China

[7] Univ Pittsburgh, Sch Med, Dept Med, Acute Lung Injury Ctr Excellence,Div Pulm Allergy, Pittsburgh, PA 15213 USA

[8] Univ Alabama Birmingham, Sch Med, Dept Biomed Engn, Birmingham, AL 35294 USA

[9] Univ Alabama Birmingham, Sch Engn, Birmingham, AL 35294 USA

[10] South Cent Univ Nationalities, Wuhan Inst Neurosci & Engn, Wuhan 430074, Hubei, Peoples R China

[11] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China

[12] Augusta Univ, Dept Pharmacol & Toxicol, Med Coll Georgia, Augusta, GA 30912 USA

[13] Univ Calif Los Angeles, Jonsson Comprehens Canc Ctr, Los Angeles, CA 90024 USA

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

基金：

中国国家自然科学基金;

关键词：

INHIBITION; RELAXATION; CHANNELS; CALCIUM;

D O I：

10.1038/s41598-018-20409-x

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Because of the serious side effects of the currently used bronchodilators, new compounds with similar functions must be developed. We screened several herbs and found that Polygonum aviculare L. contains ingredients that inhibit the precontraction of mouse and human airway smooth muscle (ASM). High K+-induced precontraction in ASM was completely inhibited by nifedipine, a selective blocker of L-type voltage-dependent Ca2+ channels (LVDCCs). However, nifedipine only partially reduced the precontraction induced by acetylcholine chloride (ACH). Additionally, the ACH-induced precontraction was partly reduced by pyrazole-3 (Pyr3), a selective blocker of TRPC3 and stromal interaction molecule (STIM)/Orai channels. These channel-mediated currents were inhibited by the compounds present in P. aviculare extracts, suggesting that this inhibition was mediated by LVDCCs, TRPC3 and/or STIM/Orai channels. Moreover, these channel-mediated currents were inhibited by quercetin, which is present in P. aviculare extracts. Furthermore, quercetin inhibited ACH-induced precontraction in ASM. Overall, our data indicate that the ethyl acetate fraction of P. aviculare and quercetin can inhibit Ca2+-permeant LVDCCs, TRPC3 and STIM/Orai channels, which inhibits the precontraction of ASM. These findings suggest that P. aviculare could be used to develop new bronchodilators to treat obstructive lung diseases such as asthma and chronic obstructive pulmonary disease.

引用

页数：12

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