Sperm processing by swim-up and density gradient is effective in elimination of sperm with DNA damage

被引：89
作者：
Jayaraman, Varshini [1 ]
Upadhya, Dinesh [1 ]
Narayan, Pratap Kumar [1 ]
Adiga, Satish Kumar [1 ]
机构：
[1] Manipal Univ, Div Reprod Med, Clin Embryol Lab, Kasturba Med Coll, Manipal 576104, Karnataka, India
来源：
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 2012年 / 29卷 / 06期
关键词：
Sperm DNA damage; Sperm selection; Semen preparation; Swim-up; Density gradient; HUMAN-SPERMATOZOA; CENTRIFUGATION; CHROMATIN; PERCOLL; FRAGMENTATION; ANTIOXIDANTS; MORPHOLOGY; INTEGRITY; ASSAY;
D O I：
10.1007/s10815-012-9742-x
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
DNA damage may occur during sperm processing, thereby negatively influencing fertilizing ability of the sperm. The present study was designed to compare the effectiveness of gradient and swim-up, either alone or in combination, to eliminate sperm with DNA damage. A total of 51 subjects visiting the University infertility clinic with normozoospermic parameters, oligozoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria; Terminal deoxy nucelotidyl transferase mediated dUTP nick end labeling assay was employed for DNA damage assessment. The percentage of TUNEL positive sperm after sperm processing was significantly lower in normozoospermic (P < 0.05), oligozoospermic (P < 0.001) and teratozoospermic samples (P < 0.01). No difference was observed in the incidence of TUNEL positive sperm between the various techniques, suggesting that they are comparable. Sperm preparation has been found to result in enrichment of sperm with intact chromatin, which is likely to improve the chances of achieving a viable pregnancy.
引用
收藏
页码：557 / 563
页数：7
相关论文
共 33 条
[1]   Sperm preparation: DNA damage by comet assay in normo- and teratozoospermics [J].
Ahmad, Laiq ;
Jalali, Samina ;
Shami, Saflad Aslam ;
Akram, Zertashia .
ARCHIVES OF ANDROLOGY, 2007, 53 (06) :325-338
[2]   ANALYSIS OF SPERM MOVEMENT IN RELATION TO THE OXIDATIVE STRESS CREATED BY LEUKOCYTES IN WASHED SPERM PREPARATIONS AND SEMINAL PLASMA [J].
AITKEN, RJ ;
BUCKINGHAM, DW ;
BRINDLE, J ;
GOMEZ, E ;
BAKER, HWG ;
IRVINE, DS .
HUMAN REPRODUCTION, 1995, 10 (08) :2061-2071
[3]  
AITKEN RJ, 1988, J ANDROL, V9, P367
[4]  
[Anonymous], 1999, WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction
[5]   Semen preparation techniques for intrauterine insemination [J].
Boomsma, C. M. ;
Heineman, M. J. ;
Cohlen, B. J. ;
Farquhar, C. .
COCHRANE DATABASE OF SYSTEMATIC REVIEWS, 2007, (04)
[6]  
Boomsma CM, 2004, COCHRANE DB SYST REV, V3
[7]  
Bormann CL., 2008, FERTIL STERIL, V90, pS452
[8]   Sperm DNA integrity assessment in prediction of assisted reproduction technology outcome [J].
Bungum, M. ;
Humaidan, P. ;
Axmon, A. ;
Spano, M. ;
Bungum, L. ;
Erenpreiss, J. ;
Giwercman, A. .
HUMAN REPRODUCTION, 2007, 22 (01) :174-179
[9]   Sperm chromatin structure assay parameters measured after density gradient centrifugation are not predictive for the outcome of ART [J].
Bungum, Mona ;
Spano, Marcello ;
Humaidan, Peter ;
Eleuteri, Patrizia ;
Rescia, Michele ;
Giwercman, Aleksander .
HUMAN REPRODUCTION, 2008, 23 (01) :4-10
[10]   COMPARISON BETWEEN A 2-LAYER DISCONTINUOUS PERCOLL GRADIENT AND SWIM-UP FOR SPERM PREPARATION ON NORMAL AND ABNORMAL SEMEN SAMPLES [J].
CHEN, SU ;
HO, HN ;
CHEN, HF ;
CHAO, KH ;
LIN, HR ;
HUANG, SC ;
LEE, TY ;
YANG, YS .
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 1995, 12 (10) :698-703
1234