A strong promoter, PMagpd, provides a tool for high gene expression in entomopathogenic fungus, Metarhizium acridum

被引：17
作者：
Cao, Yueqing [1 ,2 ]
Jiao, Run [1 ,2 ]
Xia, Yuxian [1 ,2 ]
机构：
[1] Chongqing Univ, Genet Engn Res Ctr, Coll Bioengn, Chongqing Engn Res Ctr Fungal Insecticides, Chongqing 400030, Peoples R China
[2] Chongqing Municipal Educ Commiss, Key Lab Funct Gene & Regulat Technol, Chongqing 400030, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2012年 / 34卷 / 03期
关键词：
Fungi; Gene expression; Glyceraldehyde-3-phosphate dehydrogenase; Metarhizium acridum; Promoter; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE GENE; NIDULANS GPDA GENE; BEAUVERIA-BASSIANA; TRANSFORMATION; ANISOPLIAE; RESISTANCE; CLONING; MARKER;
D O I：
10.1007/s10529-011-0805-3
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A glyceraldehyde-3-phosphate dehydrogenase gene (gpd) promoter (PMagpd) was obtained from Metarhizium acridum and its active region analyzed by 5'-deletion strategy using beta-glucuronidase (GUS) as a reporter. Sequence analysis revealed that typical regulatory elements of PMagpd were included in the 1.7 kb region upstream of the start codon of the Magpd gene. Deletion of the region from - 1,691 bp to -1,463 bp, where the gpd box is harbored, did not significantly affect the PMagpd activity. Deletions of the regions upstream of -946 bp and upstream of -684 bp caused a major decrease of GUS activity. Compared with PgpdA (2.2 kb) in Aspergillus nidulans, PMagpd (1.4 kb) had a shorter sequence and significantly higher activity in M. acridum. This study provides an applicable promoter for overexpression of target genes in M. acridum.
引用
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页码：557 / 562
页数：6
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