Age related changes in cell stiffness of tendon stem/progenitor cells and a rejuvenating effect of ROCK-inhibition

被引：24
作者：
Kiderlen, Stefanie [1 ,2 ,3 ]
Polzer, Christoph [2 ,4 ]
Raedler, Joachim O. [2 ,3 ]
Docheva, Denitsa [5 ]
Clausen-Schaumann, Hauke [1 ,3 ]
Sudhop, Stefanie [1 ,3 ]
机构：
[1] Munich Univ Appl Sci, Ctr Appl Tissue Engn & Regenerat Med CANTER, Munich, Germany
[2] Ludwig Maximilians Univ Munchen, Fac Phys, Soft Condensed Matter, Munich, Germany
[3] Ludwig Maximilians Univ Munchen, Ctr NanoSci, Munich, Germany
[4] Munich Univ Appl Sci, Multiphoton Imaging Lab, Munich, Germany
[5] Univ Regensburg, Dept Trauma Surg, Expt Trauma Surg, Med Ctr, Regensburg, Germany
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2019年 / 509卷 / 03期
关键词：
hTSPC; Elastic modulus; Cell aging; ROCK-inhibition; Rejuvenation; MESENCHYMAL STEM-CELLS; MECHANICAL-PROPERTIES; SELF-RENEWAL; IN-VITRO; FIBROBLASTS; ORGANIZATION; ELASTICITY; SENESCENCE; GROWTH;
D O I：
10.1016/j.bbrc.2019.01.027
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Tendon stem/progenitor cells (TSPC) are potential targets for regenerative medicine and the treatment of tendon injuries. The frequency of such injuries increases in elderly patients while the proportion of functional TSPCs in tendon tissue decreases, protracting tendon repair. Using atomic force microscopy (AFM), we show that cell stiffness and size increase in TSPCs isolated from elderly patients (A-TSPC) compared to TSPCs from younger patients (Y-TSPC). Additionally, two-photon excited fluorescence (TPEF) microscopy revealed a denser, well-structured actin cytoskeleton in A-TSPC, which correlates with the augmented cell stiffness. Treating A-TSPC with ROCK-inhibitor, reverses these age-related changes, and has rejuvenating effect on cell morphology and stiffness. We assume that cellular stiffness is a suitable marker for cell aging and ROCK a potential target for therapeutic applications of cell rejuvenation. (C) 2019 The Authors. Published by Elsevier Inc.
引用
收藏
页码：839 / 844
页数：6
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