Effect of high hydrostatic pressure on the porin OmpC from Escherichia coli

被引:0
|
作者
Macdonald, AG
Martinac, B
机构
[1] Univ Aberdeen, Dept Biomed Sci, Aberdeen AB24 2TZ, Scotland
[2] Univ Western Australia, Queen Elizabeth II Med Ctr, Dept Pharmacol, Nedlands, WA 6907, Australia
关键词
porin; high hydrostatic pressure; channel gating;
D O I
10.1016/S0378-1097(99)00091-9
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Porin OmpC from Escherichia coli was reconstituted in liposomes and its gating kinetics were recorded at high hydrostatic pressure, up to 90 MPa, using a development of the patch clamp technique. The composition of the recording solution influenced the results but generally high hydrostatic pressure favoured channel opening. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:327 / 334
页数:8
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